LIGAND EFFECTS ON THE LIMITED PROTEOLYSIS OF PHENYLALANINE-HYDROXYLASE - EVIDENCE FOR MULTIPLE CONFORMATIONAL STATES
LIGAND EFFECTS ON THE LIMITED PROTEOLYSIS OF PHENYLALANINE-HYDROXYLASE - EVIDENCE FOR MULTIPLE CONFORMATIONAL STATES
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DOI：
10.1016/0006-291x(83)91050-1
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
KAUFMAN, S
中科院分区：
文献类型：
--
作者：
PHILLIPS, RS;IWAKI, M;KAUFMAN, S
本文研究了苯丙氨酸和四氢生物蝶呤对胰凝乳蛋白酶限制性水解大鼠肝苯丙氨酸羟化酶的影响。四氢生物蝶呤的存在抑制天然苯丙氨酸羟化酶的蛋白水解活化。相反，苯丙氨酸在这些条件下引起蛋白水解活化的刺激。苯丙氨酸和四氢生物蝶呤都不影响胰凝乳蛋白酶对合成底物的水解速率。四氢生物蝶呤和苯丙氨酸都抑制[32 P]磷酸化苯丙氨酸羟化酶释放可溶性放射性。证实了苯丙氨酸羟化酶存在多种构象状态。
The effects of phenylalanine and tetrahydrobiopterin on the limited proteolysis of rat liver phenylalanine hydroxylase by chymotrypsin were examined. The presence of tetrahydrobiopterin inhibits the proteolytic activation of native phenylalanine hydroxylase. In contrast, phenylalanine causes a stimulation of proteolytic activation under these conditions. Neither phenylalanine nor tetrahydrobiopterin affect the rate of hydrolysis of a synthetic substrate by chymotrypsin. Both tetrahydrobiopterin and phenylalanine inhibit the release of soluble radioactivity from [32P]phosphorylated phenylalanine hydroxylase. The existence of multiple conformational states of phenylalanine hydroxylase is confirmed.