Simple and rapid quantification of serotonin transporter binding using [11C] DASB bolus plus constant infusion
Simple and rapid quantification of serotonin transporter binding using [11C] DASB bolus plus constant infusion
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DOI：
10.1016/j.neuroimage.2017.01.050
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发表时间：
2017-04-01
期刊：
影响因子：
5.7
通讯作者：
Lanzenberger, R.
中科院分区：
文献类型：
--
作者：
Gryglewski, G.;Rischka, L.;Lanzenberger, R.
简介：在人脑中5-羟色胺转运体（SERT）的体内定量一直是神经精神疾病领域分子成像的支柱，并有助于探索几种医疗条件，治疗和环境影响的基础。PET/MR混合系统的出现和SERT结合的异质性要求开发有效的方法，使得在有限的扫描时间和分子和功能措施的同时变化的情况下调查较大或脆弱的人群成为可能。我们提出[C-11]DASB推注加恒定输注这些应用程序，并验证它对标准分析的动态PET data.Methods：[C-11]DASB推注/输注优化后，在8名健康受试者[C-11]DASB推注采集的数据进行。随后，16名受试者接受了一次使用[C-11]DASB推注加K-bol恒定输注160-179分钟的扫描，以及一次在[C-11]DASB推注后进行的扫描，以进行方法间可靠性分析。对所有扫描进行动脉血样采集和代谢物分析。在9名受试者中，使用Logan图获得推注扫描的分布容积（V-T）以及不同扫描时间跨度（示踪剂输注开始后VT-70持续60-70 min，VT-90持续75-90 min，VT-120持续100-120 min）的推注加输注扫描的组织和血浆母体活性之间的比值。省略血液数据，结合电位（BPND）获得使用多线性参考组织模型（MRTM 2）和小脑灰质作为参考regions.Results：一个K-bol的160分钟被认为是最佳的快速平衡在丘脑和纹状体。VT-70在丘脑、纹状体和嗅觉皮层显示了良好的组内相关系数（ICC），为0.61-0.70，且存在偏倚
Introduction: In-vivo quantification of serotonin transporters (SERT) in human brain has been a mainstay of molecular imaging in the field of neuropsychiatric disorders and helped to explore the underpinnings of several medical conditions, therapeutic and environmental influences. The emergence of PET/MR hybrid systems and the heterogeneity of SERT binding call for the development of efficient methods making the investigation of larger or vulnerable populations with limited scanner time and simultaneous changes in molecular and functional measures possible. We propose [C-11]DASB bolus plus constant infusion for these applications and validate it against standard analyses of dynamic PET data.Methods: [C-11]DASB bolus/infusion optimization was performed on data acquired after [C-11]DASB bolus in 8 healthy subjects. Subsequently, 16 subjects underwent one scan using [C-11]DASB bolus plus constant infusion with K-bol 160-179 min and one scan after [C-11]DASB bolus for inter-method reliability analysis. Arterial blood sampling and metabolite analysis were performed for all scans. Distribution volumes (V-T) were obtained using Logan plots for bolus scans and ratios between tissue and plasma parent activity for bolus plus infusion scans for different time spans of the scan (VT-70 for 60-70 min after start of tracer infusion, VT-90 for 75-90 min, VT-120 for 100-120 min) in 9 subjects. Omitting blood data, binding potentials (BPND) obtained using multilinear reference tissue modeling (MRTM2) and cerebellar gray matter as reference region were compared in 11 subjects.Results: A K-bol of 160 min was observed to be optimal for rapid equilibration in thalamus and striatum. VT-70 showed good intraclass correlation coefficients (ICCs) of 0.61-0.70 for thalamus, striatal regions and olfactory cortex with bias