Exploring the basis of 2-propenyl and 3-butenyl glucosinolate synthesis by QTL mapping and RNA-sequencing in Brassica juncea
Exploring the basis of 2-propenyl and 3-butenyl glucosinolate synthesis by QTL mapping and RNA-sequencing in Brassica juncea
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通过 QTL 作图和 RNA 测序探索芥菜中 2-丙烯基和 3-丁烯基芥子油苷合成的基础
DOI：
10.1371/journal.pone.0220597
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发表时间：
2019-10-18
期刊：
影响因子：
3.7
通讯作者：
Tian, Entang
中科院分区：
文献类型：
--
作者：
Khattak, Aimal Nawaz;Wang, Tianya;Tian, Entang
Brassica Juncea用作调味品，作为蔬菜和油料种子，尤其是在半干旱地区。在本研究中，我们使用B. juncea的一个重组近交系（RIL）构建了一个遗传图。总共将304个ILP（内含子长度多态性）标记映射到B. Juncea中指定LG01-LG18的18个连锁组。构造的地图覆盖了1671.13 cm的总遗传长度，平均标记间隔为5.50 cm。与QTL一起在2016年和2017的野外实验中，LG08的QTL与QTL共定位的2-苯基葡萄糖醇（GSL）（GSLS）在LG08上的LG08上的QTL。 GSL分别。此外，使用Illumina RNA序列技术来挖掘负责在RIL映射种群的亲属线的小孔中合成GSL的基因，因为种子GSL的大部分可能源于分子。基因和基因组（KEGG）的基因本体论（GO）和京都百科全书的比较分析和注释表明，GSL代谢有324个基因参与GSL代谢，其中只有24个转录本是不同表达的基因（DEGS）。在这些DEG中，脂肪族GSL的生物合成和转运涉及15个基因，通过QRT-PCR分析进一步验证了它们的表达模式。联合QTL映射和RNA-sTERE-STER-STER-STER-STERITION分析显示，B. Juncea中GSL代谢的IIL1（LOC106416451）的一个候选基因。这些结果将有助于在B. juncea中的2-丙烯基和3-丁烯基GSL的进一步绘制，基因克隆和遗传机制。
Brassica juncea is used as a condiment, as vegetables and as an oilseed crop, especially in semiarid areas. In the present study, we constructed a genetic map using one recombinant inbred line (RIL) of B. juncea. A total of 304 ILP (intron length polymorphism) markers were mapped to 18 linkage groups designated LG01-LG18 in B. juncea. The constructed map covered a total genetic length of 1671.13 cM with an average marker interval of 5.50 cM. The QTLs for 2-propenyl glucosinolates (GSLs) colocalized with the QTLs for 3-butenyl GSLs between At1g26180 and BnapPIP1580 on LG08 in the field experiments of 2016 and 2017. These QTLs accounted for an average of 42.3% and 42.6% phenotypic variation for 2-propenyl and 3-butenyl GSLs, respectively. Furthermore, the Illumina RNA-sequencing technique was used to excavate the genes responsible for the synthesis of GSLs in the siliques of the parental lines of the RIL mapping population, because the bulk of the seed GSLs might originate from the siliques. Comparative analysis and annotation by gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) revealed that 324 genes were involved in GSL metabolism, among which only 24 transcripts were differentially expressed genes (DEGs). Among those DEGs, 15 genes were involved in the biosynthesis and transport of aliphatic GSLs, and their expression patterns were further validated by qRT-PCR analysis. These RNA-Seq results will be helpful for further fine mapping, gene cloning and genetic mechanisms of 2-propenyl and 3-butenyl GSLs in B. juncea.