Short-term changes in levels of cyclic AMP, adenylate cyclase, and phosphodiesterase during the initiation of sperm motility in rainbow trout.
Short-term changes in levels of cyclic AMP, adenylate cyclase, and phosphodiesterase during the initiation of sperm motility in rainbow trout.
复制标题
虹鳟鱼精子运动启动过程中环磷酸腺苷、腺苷酸环化酶和磷酸二酯酶水平的短期变化。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
K. Ishida
中科院分区：
文献类型：
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作者：
M. Morisawa;K. Ishida
为了阐明产生和降解环状AMP的系统在鲑鱼精子运动启动过程中的作用，测定了精子内环状AMP、腺苷环化酶和磷酸二酯酶水平的短期变化。将精子移入无K+培养基后1s，环磷酸腺苷和腺苷环化酶活力升高并达到最大值，随后迅速下降。然而，在富含K+的培养液中，保持静止的精子的这两个参数都没有变化。此外，磷酸二酯酶活性的升高比环磷酸腺苷和腺苷环化酶水平的升高晚4s。这些发现表明，在产卵瞬间，通过激活腺苷酸环化酶和磷酸二酯酶，细胞内环磷酸腺苷的水平在1秒内发生非常迅速的变化，并调节鲑鱼精子运动的启动。
In order to clarify the role of the system that generates and degrades cyclic AMP during the initiation of motility of trout sperm, short-term changes in levels of intraspermatozoal cyclic AMP, adenylate cyclase, and phosphodiesterase were measured. Levels of cyclic AMP and the activity of adenylate cyclase increased and reached a maximum level 1 sec after transfer of sperm to K+-free medium, where they became motile, and then decreased rapidly. However, there were no changes in either parameter in sperm which remained immotile in K+-rich medium. In addition, an increase in the activity of phosphodiesterase was observed 4 sec later than the increase in levels of cyclic AMP and adenylate cyclase. These findings suggest that a very rapid change in the level of intracellular cyclic AMP occurs within 1 sec, at the moment of spawning, by the activation of adenylate cyclase and phosphodiesterase, and regulates the initiation of trout sperm motility.