Estrogen production by luteal cells isolated from rhesus monkeys during the menstrual cycle: correlation with spontaneous luteolysis.
Estrogen production by luteal cells isolated from rhesus monkeys during the menstrual cycle: correlation with spontaneous luteolysis.
复制标题
月经周期期间从恒河猴分离的黄体细胞产生雌激素：与自发黄体溶解的相关性。
作者：
R. Stouffer;Laura A. Bennett;G. D. Hodgen
为了评估灵长类动物的黄体溶解可能是黄体分泌雌激素的结果，我们在月经周期不同阶段的恒河猴黄体中分离出的黄体细胞悬液中检测了雌二醇 (E2)、雌酮 (E1) 和黄体酮 (P) 的产生情况。在月经周期的第 16-19 天（排卵前 LH 峰值后 5-7 天）、第 21-22 天（LH 激增后 8-10 天）和第 23-25 天（LH 激增后 11-14 天）获得细胞。在基础条件下（无外源激素），第 16-19 天的细胞在体外长达 6 小时内分泌皮克量（每 5 × 104 细胞/ml 营养培养基）的 E2 和 E1，而 P 则为纳克量。仅 0.1 ng/ml hCG 即可显着 (P < 0.05) 增强第 16-19 天细胞的雌激素产生，最大反应为 100 ng/ml。在基础条件下，第 16-19 天的细胞产生的 E2 数量明显多于 E1，而第 21-22 天的细胞合成的 E2 数量相似。
To assess the hypothesis that luteolysis in primates may occur as a consequence of estrogen secretion by the corpus luteum, the production of estradiol (E2), estrone (E1), and progesterone (P) was examined in suspensions of luteal cells isolated from corpora lutea of rhesus monkeys at various stages of the menstrual cycle. Cells were obtained on days 16–19 (5-7 days after the preovulatory LH peak), days 21-22 (8-10 days after the LH surge), and days 23-25 (11-14 days after the LH surge) of the menstrual cycle. Under basal conditions (no exogenous hormones), day 16–19 cells secreted picogram quantities (per 5 × 104 cells/ml nutrient medium) of E2 and E1 compared to nanogram amounts of P for up to 6 h in vitro. Estrogen production by day 16–19 cells was significantly (P < 0.05) enhanced by as little as 0.1 ng/ml hCG, with a maximal response at 100 ng/ml. Under basal conditions, day 16–19 cells produced significantly greater amounts of E2 than E1, whereas day 21-22 cells synthesized similar quantitites of th...