Preservation of vascular function in rat mesenteric resistance arteries following cold storage, studied by small vessel myography
Preservation of vascular function in rat mesenteric resistance arteries following cold storage, studied by small vessel myography
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DOI：
10.1038/sj.bjp.0701768
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发表时间：
1998-04-01
影响因子：
7.3
通讯作者：
Hadoke, PWF
中科院分区：
文献类型：
--
作者：
McIntyre, CA;Williams, BC;Hadoke, PWF
1使用分离的血管来研究血管系统的生理和药物控制受到使用新分离的血管的要求的限制。因此，本研究的目的是确定将阻力动脉保存在4℃生理盐溶液(PSS)中是否能保护血管的平滑肌和内皮细胞功能。血管节段安装在小血管肌图仪上，用于测量等长张力，并在其最佳静止力下保持平衡。对去甲肾上腺素(NA；1x10(-9)-3x10(-5)M)、苯肾上腺素(PE，1x10(-9)-3x10(-5)M)、氯化钾(KCl2.5-140 mm)和内皮素(ET-1，1x10(-11)-3x10(-7)M)的收缩反应和对乙酰胆碱(ACh；1x10(-9)-3x10(-5)M)和3-吗啡磺酸二亚胺(SIN-1；分离后即刻(0d)和保存1~4d(1d~4d)的动脉分别获得1x10(-9)-1x10(-4)M。3所有动脉对每种血管收缩药均产生浓度依赖性收缩。血管收缩反应的幅度或敏感性(PD(2))在新鲜和储存的血管之间没有显著差异。4动脉被NA预收缩到最大反应的80%左右，对ACh和SIN-1的反应呈浓度依赖性地松弛。储存3天的血管对ACh或SIN-1.5的反应没有变化。储存4天后的分析显示，血管对ACh和SIN-1的敏感性显著增加(-logic(50)(M)值；ACh；第0天，7.46+/-0.13比第4天，7.97+/-0.11，P
1 The use of isolated blood vessels to investigate the physiological and pharmacological control of the vasculature is limited by the requirement to use freshly isolated vessels. Hence, the aim of this study was to determine whether vascular smooth muscle and endothelial cell function could be preserved in resistance arteries by storing them in physiological salt solution (PSS) at 4 degrees C.2 Third order mesenteric resistance arteries (mean internal diameter 237+/-6 mu m) were dissected from the mesenteric bed of male Cob-Wistar rats. The vessel segments were mounted in a small vessel myograph for measurement of isometric tension, and equilibrated at their optimum resting force. Contractile responses to noradrenaline (NA; 1x10(-9)-3x10(-5) M), phenylephrine (PE, 1x10(-9)-3 x10(-5) M), potassium chloride (KCl; 2.5-140 mM) and endothelin (ET-1, 1x10(-11)-3x10(-7) M) and relaxant responses to acetylcholine (ACh; 1x10(-9)-3x10(-5) M) and 3-morpholinosydnonimine (SIN-1; 1x10(-9)-1x10(-4) M) were obtained in arteries, immediately after dissection (day 0) and following one to four days storage (day 1-day 4).3 All arteries produced concentration-dependent contractions in response to each of the vasoconstrictors. There were no significant differences in the magnitude or sensitivity (pD(2)) of the vasoconstrictor responses between fresh and stored vessels.4 Arteries precontracted with NA to approximately 80% of the maximum response, relaxed in a concentration-dependent manner in response to ACh and SIN-1. Vessel storage for up to three days resulted in no change in response to ACh or SIN-1.5 Vessels analysed after four days of storage demonstrated a significant increase in sensitivity to ACh and SIN-1 (-logIC(50) (M) values; ACh; day 0, 7.46+/-0.13 vs day 4, 7.97+/-0.11, P