Uncoupling of chondrocyte death and vascular invasion in mouse galectin 3 null mutant bones
Uncoupling of chondrocyte death and vascular invasion in mouse galectin 3 null mutant bones
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DOI：
10.1006/dbio.2000.9933
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Poirier, F
中科院分区：
文献类型：
--
作者：
Colnot, C;Sidhu, SS;Poirier, F
半乳糖凝集素3是一种β-半乳糖苷结合蛋白，其定位于发育中的长骨的生长板软骨中的增殖、成熟和肥大软骨细胞的细胞质。为了阐明半乳糖凝集素3在骨发育过程中的功能，我们检查了携带半乳糖凝集素3基因无效突变的胎鼠的骨骺股骨和胫骨。详细的组织学和超微结构研究发现增生区、成熟区和肥大区的细胞和肥大区的细胞外基质中存在异常，以及肥大软骨细胞总数减少。分析了几种软骨细胞和骨细胞标志物的表达模式，并揭示了半乳糖凝集素3突变体生长板中Ihh表达的微妙修饰。在软骨血管连接处观察到显著差异，此处存在许多空陷窝。此外，在晚期肥大区发现大量凝聚的软骨细胞表现出细胞死亡的特征性迹象，表明突变体中软骨细胞死亡率增加。这些结果表明半乳糖凝集素3作为软骨细胞存活的调节剂的作用。此外，这种独特的表型表明软骨细胞的消除和血管侵袭可以解偶联，并表明半乳糖凝集素3可能在软骨细胞死亡和干骺端血管化之间的协调中发挥作用。(C)北京：科学出版社.
Galectin 3 is a beta -galactoside binding protein which localizes to the cytoplasm of proliferative, mature, and hypertrophic chondrocytes in the growth plate cartilage of developing long bones. To elucidate the function of galectin 3 during bone development, we examined the epiphyseal femurs and tibias of fetal mice carrying a null mutation for the galectin 3 gene. Detailed histological and ultrastructural studies identified abnormalities in the cells of the proliferative, mature, and hypertrophic zones and in the extracellular matrix of the hypertrophic zone, as well as a reduction in the total number of hypertrophic chondrocytes. The expression patterns of several chondrocyte and bone cell markers were analyzed and revealed a subtle modification of Ihh expression in the galectin 3 mutant growth plate. A striking difference was observed at the chondrovascular junction where many empty lacunae are present. In addition, large numbers of condensed chondrocytes exhibiting characteristic signs of cell death were found in the late hypertrophic zone, indicating that the rate of chondrocyte death is increased in the mutants. These results suggest a role for galectin 3 as a regulator of chondrocyte survival. In addition, this unique phenotype shows that the elimination of chondrocytes and vascular invasion can be uncoupled and indicates that galectin 3 may play a role in the coordination between chondrocyte death and metaphyseal vascularization. (C) 2001 Academic Press.