Relationship between ethanol-induced activity and anxiolysis in the open field, elevated plus maze, light-dark box, and ethanol intake in adolescent rats.
Relationship between ethanol-induced activity and anxiolysis in the open field, elevated plus maze, light-dark box, and ethanol intake in adolescent rats.
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DOI：
10.1016/j.bbr.2014.02.032
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发表时间：
2014-05-15
影响因子：
2.7
通讯作者：
Pautassi RM
中科院分区：
文献类型：
--
作者：
Acevedo MB;Nizhnikov ME;Molina JC;Pautassi RM
目前尚不清楚乙醇诱导的开放场（OF）运动刺激是否仅仅反映了药物的精神运动刺激作用，或者这种刺激是否是由乙醇的抗焦虑特性驱动或调节的。在本研究中，青春期大鼠被给予不同剂量的乙醇或不进行治疗。他们依次在 OF、高架十字迷宫 (EPM) 和明暗盒 (LDB) 中进行评估，然后评估乙醇摄入量。目的是评估乙醇诱导的活动和抗焦虑作用之间的关系，分析乙醇摄入量与先前乙醇暴露的函数关系，并将这些装置的行为反应性与青春期的乙醇摄入量相关联。结果表明，2.5 和 3.25 g/kg 乙醇后观察到的 OF 增强探索反映了似乎相对独立于抗焦虑作用的运动刺激作用。 1.25 g/kg剂量诱导OF中的运动刺激和EPM中的抗焦虑作用，但这些作用是相对独立的。 0.5 g/kg 乙醇剂量在 EPM 中发挥显着的抗焦虑作用，但对 OF 没有刺激作用。多元回归分析表明，OF 中的直立行为频率较高、EPM 中张开臂进入的比例较高、进入 OF 中心区域的倾向较低的青少年表现出较高的乙醇摄入量。这些结果表明 OF 是测量乙醇诱导刺激的有效程序，并为表征有开始饮酒风险的青少年亚群提供信息。
It is yet unclear if ethanol-induced motor stimulation in the open field (OF) merely reflects psychomotor stimulating effects of the drug or if this stimulation is driven or modulated by ethanol’s antianxiety properties. In the present study, adolescent rats were administered with different ethanol doses or remained untreated. They were sequentially assessed in the OF, elevated plus maze (EPM), and light-dark box (LDB) and then assessed for ethanol intake. The aims were to assess the relationship between measures of ethanol-induced activity and anxiolysis, analyze ethanol intake as a function of prior ethanol exposure, and associate behavioral responsiveness in these apparatus with ethanol intake during adolescence. The results suggested that the enhanced exploration of the OF observed after 2.5 and 3.25 g/kg ethanol reflected a motor-stimulating effect that appeared to be relatively independent of anxiolysis. The 1.25 g/kg dose induced motor stimulation in the OF and anti-anxiety effects in the EPM, but these effects were relatively independent. The 0.5 g/kg ethanol dose exerted significant anxiolytic effects in the EPM in the absence of stimulating effects in the OF. A multivariate regression analysis indicated that adolescents with a higher frequency of rearing behavior in the OF, higher percentage of open arm entries in the EPM, and lower propensity to enter the central area of the OF exhibited greater ethanol intake. These results indicate that the OF is a valid procedure for the measurement of ethanol-induced stimulation, and provide information towards characterizing subpopulations of adolescents at risk for initiating alcohol drinking.