Individual variability in the human metabolism of an arsenic-containing carbohydrate, 2',3'-dihydroxypropyl 5-deoxy-5-dimethylarsinoyl-beta-D-riboside, a naturally occurring arsenical in seafood.
Individual variability in the human metabolism of an arsenic-containing carbohydrate, 2',3'-dihydroxypropyl 5-deoxy-5-dimethylarsinoyl-beta-D-riboside, a naturally occurring arsenical in seafood.
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含砷碳水化合物 2,3-二羟丙基 5-脱氧-5-二甲基胂酰基-β-D-核苷（海鲜中天然存在的砷）的人体代谢存在个体差异。
DOI：
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发表时间：
2009
影响因子：
4.1
通讯作者：
K. Francesconi
中科院分区：
文献类型：
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作者：
R. Raml;G. Raber;Alice Rumpler;T. Bauernhofer;W. Goessler;K. Francesconi
我们报告了人类氧化砷糖代谢变异性的研究，涉及摄入化学合成的砷糖，并通过 HPLC 结合砷选择性质谱检测（ICPMS，电感耦合等离子体质谱法）定量测定尿液和血清中的砷代谢物。六名志愿者四天的尿砷排泄总量范围很广，从大约4-95%。尿液中存在的砷代谢物也表现出很大的变异性：高砷排泄伴随着摄入的氧化砷糖几乎完全生物转化为多种代谢物（> 10），而排泄少量砷的受试者相对于未变化的氧化砷糖及其代谢物产生了少量的代谢物。 硫代类似物。砷的主要尿代谢物是二甲基胂酸盐（DMA）和砷糖降解为 DMA 过程中可能的中间体，即二甲基胂酰乙醇（DMAE）和二甲基胂酰乙酸（DMAA），以其氧代和硫代类似物的形式存在。血清中还发现了硫代-DMAE 和硫代-DMAA，表明这些物质是在肝脏中形成的，而不是在膀胱中储存尿液时形成的。个体代谢砷糖的方式存在巨大差异，这对于从海鲜中摄入砷的风险评估具有重要意义。
We report studies on the variability in human metabolism of an oxo-arsenosugar involving the ingestion of a chemically synthesized arsenosugar and quantitative determination of the arsenic metabolites in urine and serum by HPLC coupled with arsenic-selective mass spectrometric detection (ICPMS, inductively coupled plasma mass spectrometry). The total, four-day, urinary excretion of arsenic for six volunteers ranged widely from ca. 4-95%. The arsenic metabolites present in the urine also showed great variability: high arsenic excretion was accompanied by almost complete biotransformation of the ingested oxo-arsenosugar into a multitude of metabolites (>10), whereas the subjects that excreted low amounts of arsenic produced low quantities of metabolites relative to unchanged oxo-arsenosugar and its thio-analogue. Major arsenic urinary metabolites were dimethylarsinate (DMA) and possible intermediates in the degradation of arsenosugar to DMA, namely, dimethylarsinoylethanol (DMAE) and dimethylarsinoylacetate (DMAA) present both as their oxo- and thio-analogues. Thio-DMAE and thio-DMAA were also found in blood serum indicating that these species were formed in the liver rather than on storage of the urine in the bladder. The large variability in the way individuals metabolize arsenosugars has implications for risk assessment of arsenic intake from seafood.