Effects of seed sludge on fermentative characteristics and microbial community structures in thermophilic hydrogen fermentation of starch
Effects of seed sludge on fermentative characteristics and microbial community structures in thermophilic hydrogen fermentation of starch
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DOI：
10.1016/j.ijhydene.2008.08.038
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发表时间：
2008-11
影响因子：
7.2
通讯作者：
Yohei Akutsu;Y. Li;M. Tandukar;K. Kubota;H. Harada
中科院分区：
文献类型：
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作者：
Yohei Akutsu;Y. Li;M. Tandukar;K. Kubota;H. Harada
本文研究了接种污泥对高温氢气发酵的影响。将5种不同种类的污泥分别接种到全搅拌式反应器(CSTR)中，不做任何处理。反应器以淀粉为底物，在高温(55℃)、水力停留时间(HRT)为24小时、50天的条件下平行运行。所有的反应器都实现了稳定的制氢。在接种高温消化活性污泥的反应器中，产氢量最高(2.32molH2/mol葡萄糖)。利用本研究得到的数据计算出的氢气发酵化学计量方程为：淀粉+([C6H10O5]n)→2.32nH2+2.14nCO2+0.50n乙酸酯+0.63n丁酸盐+0.11n细胞(C5H7NO2)。用聚合酶链式反应-变性凝胶梯度电泳法(PCR-DGGE)和16S rRNA基因克隆分析微生物群落结构。在所有的反应器中，最主要的是厌氧热细菌的近亲。
In this work, effects of seed sludge on thermophilic hydrogen fermentation were investigated. Five different kinds of sludge were separately inoculated in completely stirred tank reactors (CSTRs), without any pretreatment. The reactors were operated in parallel with starch as substrate under thermophilic condition (55°C) at a hydraulic retention time (HRT) of 24h for 50 days. Stable hydrogen production was achieved in all reactors. The highest hydrogen yield (2.32mol H2/mol glucose) was obtained from the reactor seeded with thermophilically digested activated sludge. The stoichiometric equation of hydrogen fermentation calculated using the data obtained in this study was expressed as follows: starch ([C6H10O5]n)→2.32n H2+2.14n CO2+0.50n acetate+0.63n butyrate+0.11n cell (C5H7NO2). Microbial community structure was analyzed by polymerase chain reaction–denaturing gel gradient electrophoresis (PCR–DGGE) and 16S rRNA gene cloning. Close relatives of the Thermoanaerobacterium were found to be the most predominated one in all reactors.