Silica-coated superparamagnetic iron oxide nanoparticles targeting of EPCs in ischemic brain injury
Silica-coated superparamagnetic iron oxide nanoparticles targeting of EPCs in ischemic brain injury
复制标题
二氧化硅包覆的超顺磁性氧化铁纳米粒子靶向缺血性脑损伤中的 EPC
DOI：
10.1016/j.biomaterials.2013.03.030
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发表时间：
2013-07-01
期刊：
影响因子：
14
通讯作者：
Yang, Guo-Yuan
中科院分区：
文献类型：
--
作者：
Li, Qianyun;Tang, Guanghui;Yang, Guo-Yuan
静脉内移植内皮祖细胞（EPCs）可减轻缺血性脑损伤。然而，较少的细胞归巢到受损部位限制了其功能。在本研究中，我们用二氧化硅包裹的超顺磁性氧化铁纳米颗粒（SiO4@SPIONs）标记EPCs，并施加外部磁场将SiO4@SPIONs标记的EPCs（SiO 4 @SPIOns-EPCs）引导到缺血的大脑半球。我们优化了SiO4@SPIONs标记剂量，该剂量对体外培养的EPCs的增殖、迁移和管腔形成无影响。在短暂性大脑中动脉闭塞（tMCAO）小鼠中，磁场处理显著增加了SiO 4 @SPIONs-EPCs在缺血半球的归巢。注射SiO 4 @SPIONs-EPCs并随后进行磁场处理显示，与未进行磁场处理的组相比，缺血灶周区域的神经行为结果改善，脑萎缩体积减少，微血管密度和VEGF表达增加（p < 0.05）。结果表明，外磁场可以引导SiO 4 @SPIONS-EPCs到达缺血区，增强治疗效果，表明磁引导SiO 4 @SPIONS-EPCs递送是一种有前途的脑缺血治疗方法。(C)2013爱思唯尔有限公司保留所有权利。
Intravenous transplantation of endothelial progenitor cells (EPCs) reduced ischemic brain injury. However, less cell homing to damaged sites limited its functions. In present study, we labeled EPCs with silica-coated superparamagnetic iron oxide nanoparticles (SiO4@SPIONs) and applied exterior magnetic field to guide SiO4@SPIONs-labeled EPCs (SiO4@SPIONs-EPCs) to the ischemic hemisphere of the brain. We optimized SiO4@SPIONs labeling dose, which did not affect proliferation, migration and tube formation of EPCs in vitro. SiO4@SPIONs-EPCs homing was greatly increased in ischemic hemisphere with magnetic field treatment in mice underwent transient middle cerebral artery occlusion (tMCAO). Injection of SiO4@SPIONs-EPCs and followed by magnetic field treatment showed improved neurobehavioral outcomes, reduced brain atrophic volume, increased microvessel density and VEGF expression in the ischemic perifocal region compared to groups without magnetic field treatment (p < 0.05). Our results demonstrated that exterior magnetic field could guide SiO4@SPIONs-EPCs to ischemic region and enhance therapeutic effect, suggesting that magnetic-guided SiO4@SPIONs-EPCs delivery is a promising approach in cerebral ischemic therapy. (C) 2013 Elsevier Ltd. All rights reserved.