Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia: transcriptomic and metabolomic landscapes reveal highly integrated physiological networks.
Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia: transcriptomic and metabolomic landscapes reveal highly integrated physiological networks.
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DOI：
10.1098/rspb.2017.0360
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发表时间：
2017-06-28
期刊：
影响因子：
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通讯作者：
Aksoy S
中科院分区：
文献类型：
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作者：
Bing X;Attardo GM;Vigneron A;Aksoy E;Scolari F;Malacrida A;Weiss BL;Aksoy S
饮食受到限制的昆虫依靠专性微生物来满足生物功能所必需的营养需求。采采蝇是非洲锥虫寄生虫的载体，专门以脊椎动物的血液为食，并含有专性内共生体 Wigglesworthia glsinidia。如果没有 Wigglesworthia，采采蝇就无法繁殖。这些共生体被保护在形成中肠相关细菌组器官的特殊细胞（细菌细胞）内。为了破译这种对采采蝇生存至关重要的共生体的核心功能，我们对细菌组进行了双RNA-seq分析，并对从正常和共生体治愈（不育）雌性中收集的细菌组和血淋巴进行了代谢组学分析。细菌细胞产生保护 Wigglesworthia 的免疫调节肽聚糖识别蛋白 (pgrp-lb) 和有助于营养物质传播的多种维生素转运蛋白 (smvt)。 Wigglesworthia 过度表达分子伴侣 (GroEL)，以增强其翻译/运输机制，并生物合成大量 B 族维生素（特别是 B1、B2、B3 和 B6 相关代谢物）以补充宿主营养不足的饮食。 Wigglesworthia 贡献的缺失会扰乱影响碳水化合物和氨基酸代谢的多种代谢途径。这些破坏影响核苷酸生物合成和代谢的依赖下游过程以及重要辅因子 S-腺苷甲硫氨酸 (SAM) 的生物合成。这种共生对话的整体基础知识凸显了开发创新病媒控制方法的新生物学目标。
Insects with restricted diets rely on obligate microbes to fulfil nutritional requirements essential for biological function. Tsetse flies, vectors of African trypanosome parasites, feed exclusively on vertebrate blood and harbour the obligate endosymbiont Wigglesworthia glossinidia. Without Wigglesworthia, tsetse are unable to reproduce. These symbionts are sheltered within specialized cells (bacteriocytes) that form the midgut-associated bacteriome organ. To decipher the core functions of this symbiosis essential for tsetse's survival, we performed dual-RNA-seq analysis of the bacteriome, coupled with metabolomic analysis of bacteriome and haemolymph collected from normal and symbiont-cured (sterile) females. Bacteriocytes produce immune regulatory peptidoglycan recognition protein (pgrp-lb) that protects Wigglesworthia, and a multivitamin transporter (smvt) that can aid in nutrient dissemination. Wigglesworthia overexpress a molecular chaperone (GroEL) to augment their translational/transport machinery and biosynthesize an abundance of B vitamins (specifically B1-, B2-, B3- and B6-associated metabolites) to supplement the host's nutritionally deficient diet. The absence of Wigglesworthia's contributions disrupts multiple metabolic pathways impacting carbohydrate and amino acid metabolism. These disruptions affect the dependent downstream processes of nucleotide biosynthesis and metabolism and biosynthesis of S-adenosyl methionine (SAM), an essential cofactor. This holistic fundamental knowledge of the symbiotic dialogue highlights new biological targets for the development of innovative vector control methods.