Nuclear receptor COUP-TFII-expressing neocortical interneurons are derived from the medial and lateral/caudal ganglionic eminence and define specific subsets of mature interneurons
Nuclear receptor COUP-TFII-expressing neocortical interneurons are derived from the medial and lateral/caudal ganglionic eminence and define specific subsets of mature interneurons
复制标题
DOI：
10.1002/cne.23186
复制
发表时间：
2013-02-01
影响因子：
2.5
通讯作者：
Yang, Zhengang
中科院分区：
文献类型：
--
作者：
Cai, Yuqun;Zhang, Qiangqiang;Yang, Zhengang
啮齿类动物的新皮质GABA能中间神经元起源于大脑皮质下前体区。大多数小鼠新皮质中间神经元来自内侧和尾侧神经节隆起(分别为MGE和CGE)和视前区(POA)。关于外侧神经节隆起(LGE)是否也会产生新的皮质间神经元尚有争议。以前的研究表明，转录因子COUP-TFII在CGE中表达；在这里，我们发现COUP-TFII也在背侧MGE、背侧LGE(分别为dMGE和dLGE)和POA中表达。在成年大鼠新皮质，Coup-TFII+/生长抑素(SOM)+中间神经元主要位于V层。用遗传命运图方法(Shh-Cre和Nkx2.1-Cre)证明POA和腹侧MGE不产生Coup-TFII+新皮质中间神经元，提示dMGE是COUP-TFII+/SOM+新皮质中间神经元的来源。在胚胎后期，我们还观察到COUP-TFII+/SP8+细胞从dLGE和CGE向新皮质的迁移，主要通过脑室下带。用BrdU标记dLGE前体细胞的切片培养实验为dLGE产生新的皮质中间神经元提供了进一步的证据。较早出生的dMGE来源的Coup-TFII+中间神经元占据皮层V层，较晚出生的dLGE-和CGE来源的Coup-TFII+中间神经元优先占据皮质浅层。胚胎期为14.5dMGE和E15.5dLGE的新生儿移植后也观察到类似的板层分布。这些结果提供了有关神经节隆起在空间和时间上不同起源的神经元间命运和位置的新信息。J.Comp.神经。521：479497,2013年。(C)2012年威利期刊公司。
Neocortical GABAergic interneurons in rodents originate from subpallial progenitor zones. The majority of mouse neocortical interneurons are derived from the medial and caudal ganglionic eminences (MGE and CGE, respectively) and the preoptic area (POA). It is controversial whether the lateral ganglionic eminence (LGE) also generates neocortical interneurons. Previously it was shown that the transcription factor COUP-TFII is expressed in the CGE; here we show that COUP-TFII is also expressed in the dorsal MGE, dorsal LGE (dMGE and dLGE, respectively), and POA. In the adult neocortex, COUP-TFII+/somatostatin (SOM)+ interneurons are mainly located in layer V. Using a genetic fate-mapping approach (Shh-Cre and Nkx2.1-Cre), we demonstrate that the POA and ventral MGE do not give rise to COUP-TFII+ neocortical interneurons, suggesting that the dMGE is the source of COUP-TFII+/SOM+ neocortical interneurons. We also observed a migratory stream of COUP-TFII+/Sp8+ cells emanating from the dLGE and CGE to the neocortex mainly through the subventricular zone at later embryonic stages. Slice culture experiments in which dLGE progenitors were labeled with BrdU provided additional evidence that the dLGE generates neocortical interneurons. While earlier-born dMGE-derived COUP-TFII+ interneurons occupy cortical layer V, later-born dLGE- and CGE-derived COUP-TFII+ ones preferentially occupy superficial cortical layers. A similar laminar distribution was observed following neonatal transplantation of embryonic day (E)14.5 dMGE and E15.5 dLGE. These results provide novel information about interneuron fate and position from spatially and temporally distinct origins in the ganglionic eminences. J. Comp. Neurol. 521:479497, 2013. (c) 2012 Wiley Periodicals, Inc.