NEURONAL MAPPING - A PHOTO-OXIDATION REACTION MAKES LUCIFER YELLOW USEFUL FOR ELECTRON-MICROSCOPY

NEURONAL MAPPING - A PHOTO-OXIDATION REACTION MAKES LUCIFER YELLOW USEFUL FOR ELECTRON-MICROSCOPY
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DOI:
10.1126/science.7112109
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发表时间:
1982-01-01
期刊:
影响因子:
56.9
通讯作者:
MARANTO, AR
MARANTO, AR
中科院分区:
综合性期刊1区
文献类型:
--
作者:
MARANTO, AR

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Irradiating Lucifer yellow-filled neurons [in Hirudo virilis and Orconectes medicinalis] with intense blue light in the presence of 3,3''-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when Co or horseradish peroxidase injections are difficult or when a 2nd intracellular marker is needed to demonstrate neuronal contacts.