Biphasic subcellular localization of the DAZL-related protein boule in Drosophila spermatogenesis
Biphasic subcellular localization of the DAZL-related protein boule in Drosophila spermatogenesis
复制标题
DOI：
10.1006/dbio.1998.9098
复制
发表时间：
1998-12-15
影响因子：
2.7
通讯作者：
Wasserman, SA
中科院分区：
文献类型：
--
作者：
Cheng, MH;Maines, JZ;Wasserman, SA
果蝇boule基因仅在雄性生殖系中表达，并编码一种与哺乳动物生育因子密切相关的RNA结合蛋白，该生育因子由DAZ（无精子症）和DAZL（DAZ样）基因编码。梨晶的突变阻断了两次减数分裂。尽管如此，分化仍在继续，导致四倍体精子细胞无法成熟为精子。我们已经发现，Boule定位premeiotically到一个核仁周围的区域，然后易位到细胞质开始减数分裂。我们发现，删除Y染色体KS-1生育位点消除了布尔核定位，虽然它不干扰进入减数分裂。基于这些观察，我们提出，Boule行为在细胞质中调节稳定性或翻译的信使RNA编码的一个重要的减数分裂因子。(C)北京：科学出版社.
The Drosophila boule gene is expressed exclusively in the male germline and encodes an RNA binding protein closely related to the mammalian fertility factors encoded by the DAZ (Deleted in Azoospermia) and DAZL (DAZ-like) genes. Mutation of boule blocks both meiotic divisions. Differentiation nonetheless continues, resulting in tetraploid spermatids that fail to mature into sperm. We have found that Boule localizes premeiotically to a perinucleolar region and then translocates to the cytoplasm at the onset of meiosis. We show that deletion of the Y chromosome ks-1 fertility locus eliminates Boule nuclear localization, although it does not perturb entry into meiosis. Based on these observations we propose that Boule acts in the cytoplasm to regulate the stability or translation of messenger RNA encoding an essential meiotic factor. (C) 1998 Academic Press.