Fingerprint quality control of Tianjihuang by high-performance liquid chromatography-photodiode array detection
Fingerprint quality control of Tianjihuang by high-performance liquid chromatography-photodiode array detection
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DOI：
10.1016/j.chroma.2005.02.081
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发表时间：
2005-04-08
影响因子：
4.1
通讯作者：
Su, WW
中科院分区：
文献类型：
--
作者：
Yang, LW;Wu, DH;Su, WW
建立了一种用于治疗多种细菌性疾病、感染性肝炎、胃肠疾病、内出血和肿瘤的中草药金丝桃的高效液相色谱-二极管阵列检测指纹图谱分析方法。首次采用国家食品药品监督管理局(SFDA)推荐的专业分析软件与色谱图进行了系统的比对，考察并论证了利用指纹图谱进行天积黄药材质量评价的可行性和优越性。结果表明，采用指纹图谱与相似度评价相结合的方法可以有效地鉴别和区分不同来源的田鸡黄药材。考察了采集地点、采收时间和贮藏时间对中草药指纹图谱的影响。(C)2005 Elsevier B.V.保留所有权利。
A novel, simple and accurate fingerprint method was developed using high-performance liquid chromatography-photodiode array detection (HPLC-DAD) for the quality control of Hypericum japonicum thunb (Tianjihuang), a Chinese herbal medicine used for the treatment of several bacterial diseases, infectious hepatitis, gastrointestinal disorder, internal hemorrhage and tumors. For the first time, the feasibility and advantages of employing chromatographic fingerprint were investigated and demonstrated for the evaluation of Tianjihuang by systematically comparing chromatograms with a professional analytical software recommended by State Food and Drug Administration (SFDA). Our results revealed that the chromatographic fingerprint combining similarity evaluation could efficiently identify and distinguish raw herbs of Tianjihuang from different sources. The effects resulted from collecting locations, harvesting time and storage time on herbal chromatographic fingerprints were also examined. (c) 2005 Elsevier B.V. All rights reserved.