Phenotypic and genotypic characterization of Streptococcus pneumoniae resistant to macrolide in Casablanca, Morocco
Phenotypic and genotypic characterization of Streptococcus pneumoniae resistant to macrolide in Casablanca, Morocco
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DOI：
10.1016/j.meegid.2016.03.003
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发表时间：
2016-06-01
影响因子：
3.2
通讯作者：
Elmdaghri, Naima
中科院分区：
文献类型：
--
作者：
Diawara, Idrissa;Zerouali, Khalid;Elmdaghri, Naima
在摩洛哥，13价肺炎球菌结合疫苗（PCV-13）于2010年10月引入国家免疫规划（NIP），并于2012年7月被PCV-10取代。本研究旨在确定2007年1月至2014年12月期间摩洛哥卡萨布兰卡耐红霉素肺炎链球菌（ERSP）的流行情况，并分析这些分离株的表型和基因型特征。从卡萨布兰卡Ibn Rochd大学医院中心的微生物实验室获得分离株。使用Pneumotest试剂盒进行血清分型，并通过Quellung囊肿胀进行血清分型。药敏试验采用纸片扩散法和Etest法。总共655个S。从2007年至2014年从儿童和成人患者中收集肺炎分离株。这些分离株中有55%来自侵袭性肺炎球菌疾病。在655株分离株中，92株（14%）为ERSP。在全球范围内，2007年至2010年（疫苗接种前）和2011年至2014年（疫苗接种后）的ERSP比例分别为11.6%和17.2%（p = 0.04）。在92例ERSP中，分别有89%、4%和7%表现为组成型MLSB（对大环内酯类、林可酰胺和链阳性菌素B耐药）、诱导型MLSB和M表型（仅对大环内酯类耐药）。ERSP基因型分析显示90.2%携带ermB基因，6.5%携带mefE基因，3.3%同时携带ermB和mefE基因。接种前ERSP血清型以6 B、19 F和23 F为主，接种后以19 F、6 B、6A和23 F为主。红霉素耐药株中，肺炎在卡萨布兰卡相对较高。PCV对减少抗生素使用的贡献是令人鼓舞的，但这应该伴随着合理使用抗生素。(c)© 2016 Elsevier B. V.版权所有。
In Morocco, the 13-valent pneumococcal conjugate vaccine (PCV-13) was introduced in the national immunization program (NIP) in October 2010 and replaced by the PCV-10 in July 2012. The present study aimed to determine the prevalence of erythromycin-resistant Streptococcus pneumoniae (ERSP) and to analyze the phenotypic and genotypic characteristics of these isolates in Casablanca, Morocco from January 2007 to December 2014. Isolates were obtained from the Microbiology Laboratory of Ibn Rochd University Hospital Centre of Casablanca. Serogrouping was done using Pneumotest Kit and serotyping by the Quellung capsular swelling. Antibiotic susceptibility pattern was determined by disk diffusion and Etest methods. A total of 655 S. pneumoniae isolates were collected from 2007 to 2014 from pediatric and adult patients. Fifty-five percent of these isolates were from invasive pneumococcal diseases. Of the 655 isolates, 92 (14%) were ERSP. Globally, the proportion of ERSP from 2007 to 2010 (before vaccination) and from 2011 to 2014 (after vaccination) were 11.6% and 17.2% (p = 0.04), respectively. Of the 92 ERSP, 89%, 4% and 7% displayed constitutive MLSB (resistance to macrolide, lincosamide and streptogramin B), inducible MLSB, and M phenotype (resistance to macrolide only), respectively. ERSP genotypic analysis showed that 90.2% carried the ermB gene, 6.5% the mefE gene, and 3.3% both the genes (ermB + mefE). The most prevalent ERSP serotypes were 6B, 19F and 23F before vaccination and 19F, 6B, 6A and 23F after vaccination. Erythromycin resistance among S. pneumoniae is relatively high in Casablanca. The contribution of PCVs to the reduction in antibiotic use is encouraging but this should be accompanied by a rational use of antibiotic. (c) 2016 Elsevier B.V. All rights reserved.