Osteogenic Differentiation Evaluation of an Engineered Extracellular Matrix Based Tissue Sheet for Potential Periosteum Replacement.
Osteogenic Differentiation Evaluation of an Engineered Extracellular Matrix Based Tissue Sheet for Potential Periosteum Replacement.
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DOI：
10.1021/acsami.5b07386
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发表时间：
2015-10
影响因子：
9.5
通讯作者：
Qi Xing;Zichen Qian;B. Kannan;Mitchell Tahtinen;F. Zhao
中科院分区：
文献类型：
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作者：
Qi Xing;Zichen Qian;B. Kannan;Mitchell Tahtinen;F. Zhao
由于骨膜在骨缺损的修复和再生中起着不可或缺的作用，利用工程化仿生物骨膜来促进骨移植的成骨将是非常有益的。骨膜基质微环境由多种高度组织化的细胞外基质(ECM)纤维组成，因此从人真皮成纤维细胞膜中提取的定向天然ECM膜在人工骨膜制造中可能具有优势。人骨髓间充质干细胞(HMSCs)具有强大的成骨潜能和快速的体外扩增能力，已被用来替代天然骨膜中的成骨细胞群。本工作的目的是研究天然ECM片材和基质对位是否能促进hMSCs的体外成骨。以常规细胞培养底物I型胶原包被的聚二甲基硅氧烷(PDMS)和组织培养塑料(TCP)为对照。结果表明，ECM片剂能显著提高碱性磷酸酶活性和钙沉积。骨特异性基因表达的增加进一步证实了成骨能力的增强。细胞外基质能显著结合大量的生长因子，包括血管生成素-1、转化生长因子-β-1、碱性成纤维细胞生长因子和血管内皮生长因子，以及磷酸钙纳米颗粒，从而促进了人骨髓间充质干细胞在细胞外基质上的高成骨能力。然而，底物的排列对成骨活性和生长因子结合没有明显影响。这些结果表明hMSC种植的ECM片作为仿生骨膜在促进临界大小的骨再生方面具有巨大的潜力。
Due to the indispensable role of periosteum in bone defect healing and regeneration, a promising method to enhance osteogenesis of bone grafts by using an engineered biomimetic periosteum would be highly beneficial. The stromal microenvironment of periosteum is composed of various highly organized extracellular matrix (ECM) fibers, so an aligned natural ECM sheet, derived from the human dermal fibroblast cell sheet, may be advantageous when applied for artificial periosteum fabrication. Human mesenchymal stem cells (hMSCs) have been used to replace the osteoprogenitor cell population in native periosteum due to hMSCs' great osteogenic potential and fast in vitro expansion capacity. The objective of this work is to investigate if the natural ECM sheet and the substrate alignment can promote in vitro osteogenesis of hMSCs. The conventional cell culture substrates collagen I-coated polydimethylsiloxane (PDMS) and tissue culture plastic (TCP) were used as controls. It was found that the ECM sheet significantly increased alkaline phosphatase activity and calcium deposition. The enhanced osteogenic potential was further confirmed by increased bone-specific gene expression. The ECM sheet can bind significantly higher amounts of growth factors including ANG-1, TGF-β1, bFGF, and VEGF, as well as calcium phosphate nanoparticles, which contributed to high osteogenesis of the hMSCs on ECM sheet. However, the alignment of the substrates did not show significant influence on osteogenic activity and growth factor binding. These results demonstrated the great potential of hMSC-seeded ECM sheet as a biomimetic periosteum to improve critical sized bone regeneration.