Somatic stem cell niche tropism in Wolbachia
Somatic stem cell niche tropism in Wolbachia
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DOI：
10.1038/nature04756
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发表时间：
2006-05-25
期刊：
影响因子：
64.8
通讯作者：
Wieschaus, Eric
中科院分区：
文献类型：
--
作者：
Frydman, Horacio M.;Li, Jennifer M.;Wieschaus, Eric
沃尔巴克氏体是在所有主要节肢动物群体的生殖组织中发现的细胞内细菌（1，2）。它们以类似于线粒体遗传的模式从雌性宿主垂直传递给后代。但是沃尔巴克氏体的传播与宿主的传播不平行，这表明水平传染性传播也必须发生（3-5）。昆虫寄生虫被认为是最有可能的媒介，但水平转移的机制在很大程度上尚不清楚（4，6，7）。在这里，我们表明，新引进的沃尔巴克氏体交叉几个组织和感染的成年果蝇雌性生殖系。通过对感染过程中细菌迁移模式的研究，我们发现Wolbachia通过体干细胞小生境到达D.黑腹菌此外，我们的数据表明，沃尔巴克氏体在长期感染宿主的体干细胞生态位中非常丰富，这意味着这个位置也可能有助于有效的垂直传播。据我们所知，这是第一次报告的细胞内寄生虫显示向性的干细胞龛。
Wolbachia are intracellular bacteria found in the reproductive tissue of all major groups of arthropods(1,2). They are transmitted vertically from the female hosts to their offspring, in a pattern analogous to mitochondria inheritance. But Wolbachia phylogeny does not parallel that of the host, indicating that horizontal infectious transmission must also occur(3-5). Insect parasitoids are considered the most likely vectors, but the mechanism for horizontal transfer is largely unknown(4,6,7). Here we show that newly introduced Wolbachia cross several tissues and infect the germline of the adult Drosophila melanogaster female. Through investigation of bacterial migration patterns during the course of infection, we found that Wolbachia reach the germline through the somatic stem cell niche in the D. melanogaster germarium. In addition, our data suggest that Wolbachia are highly abundant in the somatic stem cell niche of long-term infected hosts, implying that this location may also contribute to efficient vertical transmission. This is, to our knowledge, the first report of an intracellular parasite displaying tropism for a stem cell niche.