EXPRESSION OF ADHESION MOLECULES ON CD34(+) CELLS - CD34(+) L-SELECTIN(+) CELLS PREDICT A RAPID PLATELET RECOVERY AFTER PERIPHERAL-BLOOD STEM-CELL TRANSPLANTATION
EXPRESSION OF ADHESION MOLECULES ON CD34(+) CELLS - CD34(+) L-SELECTIN(+) CELLS PREDICT A RAPID PLATELET RECOVERY AFTER PERIPHERAL-BLOOD STEM-CELL TRANSPLANTATION
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DOI：
10.1182/blood.v85.11.3313.bloodjournal85113313
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发表时间：
1995-06-01
期刊：
影响因子：
20.3
通讯作者：
VANDERSCHOOT, CE
中科院分区：
文献类型：
--
作者：
DERCKSEN, MW;GERRITSEN, WR;VANDERSCHOOT, CE
黏附分子在造血祖细胞的迁移和造血调控中起着重要作用。为探讨CD34(+)细胞的动员过程是否与黏附分子表达的变化有关，检测了骨髓CD34(+)细胞和粒细胞集落刺激因子(G-CSF)联合化疗后外周血CD34(+)细胞CD31、CD44、L-选择素、唾液酸化刘易斯(X)、β1整合素非常晚期抗原4(VLA-4)和VLA-5、β2整合素淋巴细胞功能相关1(Mac-1)的表达。β1整合素VLA-4在外周血祖细胞上的表达浓度明显低于在BM CD34(+)细胞上的表达浓度，无论是在稳态造血过程中还是在白细胞分离时都是如此。未发现其他黏附分子表达水平的差异，为深入了解黏附分子可能参与外周血干细胞归巢，对27例患者的白细胞分离材料中表达这些黏附分子的CD34(+)细胞的数量进行了量化，并与强化化疗后的造血功能恢复相关联。与CD34(+)细胞总数(r=-0.55)相比，L-选择素表达亚群中CD34(+)细胞数量与移植后血小板恢复时间的相关性更好(r=-0.86)。统计分析CD34(+)L-选择素(+)细胞数与血小板恢复的关系，得出快速恢复血小板的阈值为2.1x10(6)CD34(+)L-选择素(+)细胞/kg。接受大于或等于2.1x10(6)L-选择素(+)细胞/kg(中位数11天，范围7~16天)的15例患者中有13例血小板恢复较快(小于或等于14天)，而接受较少双阳性细胞的12例患者中有10例血小板恢复较慢(中位数20天；范围13~37天)。CD34(+)细胞L-选择素(+)亚群与中性粒细胞恢复时间(r=-0.70)的相关性也好于回输CD34(+)细胞总数(r=-0.51)，但后一种差异未达到统计学意义，提示L-选择素参与了CD34(+)细胞的归巢。(C)1995年由美国血液病学会主办。
Adhesion molecules play a role in the migration of hematopoietic progenitor cells and regulation of hematopoiesis. To study whether the mobilization process is associated with changes in expression of adhesion molecules, the expression of CD31, CD44, L-selectin, sialyl Lewis(x), beta 1 integrins very late antigen 4 (VLA-4) and VLA-5, and beta 2 integrins lymphocyte function-associated 1 and Mac-1 was measured on either bone marrow (BM) CD34(+) cells or on peripheral blood CD34(+) cells mobilized with a combination of granulocyte colony-stimulating factor (G-CSF) and chemotherapy. beta 1 integrin VLA-4 was expressed at a significantly lower concentration on peripheral blood progenitor cells than on BM CD34(+) cells, procured either during steady-state hematopoiesis or at the time of leukocytapheresis. No differences in the level of expression were found for the other adhesion molecules, To obtain insight in which adhesion molecules may participate in the homing of peripheral blood stem cells (PBSCs), the number of CD34(+) cells expressing these adhesion molecules present in leukocytapheresis material was quantified and correlated with hematopoietic recovery after intensive chemotherapy in 27 patients. The number of CD34(+) cells in the subset defined by L-selectin expression correlated significantly better with time to platelet recovery after PBSC transplantation (r = -.86) than did the total number of CD34(+) cells (r = -.55). Statistical analysis of the relationship between the number of CD34(+)L-selectin(+) cells and platelet recovery resulted in a threshold value for rapid platelet recovery of 2.1 x 10(6) CD34(+) L-selectin(+) cells/kg. A rapid platelet recovery (less than or equal to 14 days) was observed in 13 of 15 patients who received greater than or equal to 2.1 x 10(6) CD34(+) L-selectin(+) cells/kg (median, 11 days; range, 7 to 16 days), whereas 10 of 12 patients who received less double positive cells had a relative slow platelet recovery (median, 20 days; range, 13 to 37 days). The L-selectin(+) subpopulation of CD34(+) cells also correlated better with time to neutrophil recovery (r = -.70) than did the total number of reinfused CD34(+) cells (r = -.51), However, this latter difference failed to reach statistical significance, This study suggests that L-selectin is involved in the homing of CD34(+) cells after PBSC transplantation. (C) 1995 by The American Society of Hematology.