Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium
Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium
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DOI：
10.1530/rep-13-0655
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发表时间：
2014-08-01
期刊：
影响因子：
3.8
通讯作者：
Sakurai, Toshihiro
中科院分区：
文献类型：
--
作者：
Bai, Rulan;Bai, Hanako;Sakurai, Toshihiro
在双向交流之后，孕体和子宫上皮必须建立适当的细胞-细胞相互作用，从而导致着床过程的进展。为了阐明孕体附着于子宫内膜的机制，我们研究了血管细胞粘附分子（VCAM 1）是否在围附着期牛孕体或子宫内膜中表达。子宫VCAM 1的表达是最小的第17天（第0天=发情期）周期和怀孕的动物，但增加之间的第20天和第22天的怀孕。在泪阜间区域，VCAM 1蛋白定位于腔上皮和腺上皮，而在泪阜区域，VCAM 1蛋白在子宫内膜的基质和内皮中检测到。在培养的子宫内膜上皮细胞（EECs），VCAM 1的表达上调时，子宫冲洗液或生长因子处理，并进一步增加时，EECs与牛滋养层CT 1细胞共培养。CT 1细胞中VCAM 1的表达也随着子宫冲洗液的使用而上调，并且当这些细胞与EECs共培养时进一步增加。VCAM 1受体、整合素α 4（ITGA 4）mRNA的表达在孕22天显著增加。在妊娠第22天的子宫中，VCAM 1蛋白在EECs和孕体中均被发现，但ITGA 4仅定位于滋养细胞。这些观察结果表明，需要足够的VCAM 1和ITGA 4在牛种的表达孕体和子宫上皮细胞之间的细胞-细胞相互作用，并建议，子宫VCAM 1和孕体ITGA 4在建立孕体粘附到子宫内膜中发挥作用。
Following bidirectional communication, the conceptus and the uterine epithelium must establish a proper cell-cell interaction, resulting in the progression of implantation processes. To clarify the mechanism of conceptus attachment to the uterine endometrium, we studied whether vascular cell adhesion molecule (VCAM1) was expressed in bovine conceptuses or endometrium during the peri-attachment period. Uterine VCAM1 expression was minimal in day 17 (day 0=day of estrus) cyclic and pregnant animals, but increased between days 20 and 22 of pregnancy. In the intercaruncular regions, VCAM1 protein was localized to the luminal and glandular epithelia, whereas in the caruncular regions, VCAM1 protein was detected in the stroma and endothelia of the uterine endometrium. In cultured endometrial epithelial cells (EECs), VCAM1 expression was up-regulated when treated with uterine flushings or growth factor and further increased when EECs were cocultured with bovine trophoblast CT1 cells. VCAM1 expression in CT1 cells was also up-regulated with the use of uterine flushings, and further increased when these cells were cocultured with EECs. Expression of VCAM1 receptor, integrin a 4 (ITGA4) mRNA, increased significantly in day 22 conceptuses. In day 22 pregnant uteri, VCAM1 protein was found in both EECs and conceptuses, but ITGA4 was localized only to trophoblasts. These observations indicate that cell-cell interactions between conceptuses and uterine epithelial cells are required for sufficient VCAM1 and ITGA4 expression in the bovine species and suggest that uterine VCAM1 and conceptus ITGA4 play a role in the establishment of conceptus adhesion to the uterine endometrium.