Mast cell chymase decreases the barrier function and inhibits the migration of corneal epithelial cells
Mast cell chymase decreases the barrier function and inhibits the migration of corneal epithelial cells
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DOI：
10.1080/02713680500346625
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发表时间：
2005-12-01
影响因子：
2
通讯作者：
Miyazaki, M
中科院分区：
文献类型：
--
作者：
Ebihara, N;Funaki, T;Miyazaki, M
目的：研究人肥大细胞糜酶对体外培养的角膜上皮细胞的作用。研究方法：将人角膜上皮细胞与人糜酶一起孵育，其活性水平可能存在于春季角结膜炎患者的泪液中。结果：糜酶孵育导致角膜上皮屏障功能下降。Occludin蛋白被糜蛋白酶切割。在伤口试验中，与糜酶孵育导致细胞迁移的抑制。结论：人糜酶可引起闭合蛋白和纤连蛋白的蛋白水解，导致角膜上皮细胞屏障功能降低和迁移抑制。
Purpose : We investigated the in vitro effects of human mast cell chymase on corneal epithelial cells. Methods : Human corneal epithelial cells were incubated with human chymase at activity levels that were likely to exist in the tears of patients with vernal keratoconjunctivitis. Results : Incubation of chymase resulted in a decrease of barrier function of corneal epithelium. Occludin protein was cleaved by chymase. In the wound assay, incubation with chymase resulted in an inhibition of cell migration. Conclusion : Human chymase causes the proteolysis of occludin and fibronectin, resulting in a decrease of barrier function and inhibition of the migration of corneal epithelial cells.