Urinary cGMP as biological marker of the renal activity of atrial natriuretic factor.
Urinary cGMP as biological marker of the renal activity of atrial natriuretic factor.
复制标题
尿cGMP作为心房钠尿因子肾活性的生物标志物。
DOI：
10.1152/ajprenal.1988.255.6.f1220
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Cogan,MG
中科院分区：
文献类型：
--
作者：
Wong,KR;Xie,MH;Shi,LB;Liu,FY;Huang,CL;Gardner,DG;Cogan,MG
目前的证据表明鸟苷 3',5'-环单磷酸 (cGMP) 在体内充当肾脏中心房钠尿因子 (ANF) 的第二信使。我们检查了细胞外 cGMP 积累是否定量反映了肾细胞内 cGMP 的浓度，以及 cGMP 的尿排泄是否与 ANF 的生理作用相关。在肾上皮 LLC-PK1 细胞中检查了 cGMP 排出。 ANF 增强细胞内 cGMP 浓度和细胞外 cGMP 外观。细胞外 cGMP 是细胞内 cGMP 浓度的时间积分函数。在清醒大鼠的清除研究中，尿 cGMP 主要来源于肾细胞，并与 ANF 诱导的尿钠排泄呈时间依赖性和浓度依赖性相关。尿 cGMP 排泄可用作体内 ANF 肾活性的生物标志物。
Current evidence suggests guanosine 3',5'-cyclic monophosphate (cGMP) serves as the second messenger for atrial natriuretic factor (ANF) in the kidney in vivo. We examined whether extracellular cGMP accumulation quantitatively reflected the concentration of cGMP within renal cells and whether urinary excretion of cGMP correlated with the physiological action of ANF. cGMP egression was examined in renal epithelial LLC-PK1 cells. ANF augmented intracellular cGMP concentration and extracellular cGMP appearance. Extracellular cGMP was an excellent function of the time-integrated intracellular cGMP concentration. In clearance studies in awake rats, urinary cGMP was primarily of renal cellular origin and correlated with the natriuresis induced by ANF in a time-dependent and concentration-dependent fashion. Urinary cGMP excretion may be useful as a biological marker for the renal activity of ANF in vivo.