Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian.
Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian.
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DOI：
10.1016/j.devcel.2016.04.009
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发表时间：
2016-05
影响因子：
11.8
通讯作者：
N. Shibata;Makoto Kashima;Taisuke Ishiko;Osamu Nishimura;Labib Rouhana;K. Misaki;S. Yonemura;Kuniaki Saito;H. Siomi;M. Siomi;K. Agata
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作者：
N. Shibata;Makoto Kashima;Taisuke Ishiko;Osamu Nishimura;Labib Rouhana;K. Misaki;S. Yonemura;Kuniaki Saito;H. Siomi;M. Siomi;K. Agata
多能干细胞（PSC）的分化需要转座子沉默贯穿整个过程。PIWI是生殖系转座子沉默的关键因子，也已知其在真涡虫PSC（neoblast）的体细胞分化中起作用。然而，PIWI如何控制后一个过程仍然难以捉摸。在这里，使用三角洲，我们表明，核PIWI，DjPiwiB，结合到PIWI相互作用的RNA（通常是PIWI依赖的转座子沉默的关键介质），并检测到不仅在neoblasts，而且其后代体细胞，不表达PIWI。相反，胞质DjPiwiA和DjPiwiC仅在新成细胞中检测到，与它们在那里的转录雅阁。DjPiwiB对再生是不可缺少的，但对转座子沉默在neoblasts中是不可缺少的。然而，转座子在DjPiwiB敲低的涡虫分化开始时被去抑制。因此，DjPiwiB似乎由新成细胞的后代体细胞遗传，以确保那些不能产生PIWI蛋白的细胞中的转座子沉默。
Differentiation of pluripotent stem cells (PSCs) requires transposon silencing throughout the process. PIWIs, best known as key factors in germline transposon silencing, are also known to act in somatic differentiation of planarian PSCs (neoblasts). However, how PIWIs control the latter process remains elusive. Here, usingDugesia japonica, we show that a nuclear PIWI, DjPiwiB, was bound to PIWI-interacting RNAs (generally key mediators of PIWI-dependent transposon silencing), and was detected in not only neoblasts but also their descendant somatic cells, which do not expresspiwi. In contrast, cytoplasmic DjPiwiA and DjPiwiC were detected only in neoblasts, in accord with their transcription there. DjPiwiB was indispensable for regeneration, but dispensable for transposon silencing in neoblasts. However, transposons were derepressed at the onset of differentiation in DjPiwiB-knockdown planarians. Thus, DjPiwiB appears to be inherited by descendant somatic cells of neoblasts to ensure transposon silencing in those cells, which are unable to produce PIWI proteins.