A DISCRETE SUBPOPULATION OF HUMAN MONOCYTES EXPRESSES A NEUTROPHIL-LIKE PROINFLAMMATORY (P) PHENOTYPE
A DISCRETE SUBPOPULATION OF HUMAN MONOCYTES EXPRESSES A NEUTROPHIL-LIKE PROINFLAMMATORY (P) PHENOTYPE
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DOI：
10.1152/ajplung.1994.267.6.l775
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发表时间：
1994-12-01
影响因子：
4.9
通讯作者：
CAMPBELL, EJ
中科院分区：
文献类型：
--
作者：
OWEN, CA;CAMPBELL, MA;CAMPBELL, EJ
我们已经证明，一个离散的和自然发生的人类单核细胞亚群表达中性粒细胞样促炎(P)表型。P单核细胞构成循环单核细胞池的20%-30%，其特征是1)通过高水平的细胞表面表达α(5-)、β(1-)和β(2)-整合素而与细胞外基质亲切地黏附；2)高能力产生活性氧；3)高含量的丝氨酸蛋白酶和α(1)-蛋白酶抑制剂；以及4)对可溶性多肽-人类白细胞弹性蛋白酶底物、[H-3]弹性蛋白和固相纤维连接蛋白的蛋白分解活性，即使在存在蛋白酶抑制剂的情况下也是如此。然而，P单核细胞很少或根本不表达细胞表面的HLA-DR抗原，这表明它们不能参与特异性免疫反应。相比之下，其余的循环单核细胞具有较低的促炎潜能，但包含高水平表达HLA-DR抗原的单核细胞群。单核细胞可以很容易地从其余单核细胞中分离出来，因为1)单核细胞与纤维连接蛋白的黏附能力；2)当使用流式细胞仪或免疫磁珠时，P单核细胞不表达HLA-DR抗原。我们的数据表明，当被募集到炎症部位时，P单核细胞可以促进炎症的消退或促进组织损伤。
We have demonstrated that a discrete and naturally occurring subpopulation of human monocytes expresses a neutrophil-like proinflammatory (P) phenotype. P monocytes constitute 20-30% of the circulating monocyte pool and are characterized by 1) avid adherence to extracellular matrix through high-level cell-surface expression of alpha(5-), beta(1-), and beta(2)-integrins; 2) high capacity to produce reactive oxygen species; 3) high content of serine proteinases and alpha(1)-proteinase inhibitor; and 4) proteolytic activity against a soluble peptide human leukocyte elastase substrate, [H-3]elastin, and solid-phase fibronectin, even in the presence of proteinase inhibitors. However, P monocytes express little or no cell-surface HLA-DR antigen, suggesting that they are unable to participate in specific immune responses. In contrast, the remainder of circulating monocytes have a low proinflammatory potential but contain the population of monocytes with high-level expression of HLA-DR antigen. P monocytes can readily be separated from the remainder of monocytes on the basis of 1) their capacity to adhere to fibronectin; and 2) their absent expression of HLA-DR antigen when flow cytometry or immunomagnetic beads are used. Our data indicate that, when recruited to sites of inflammation, P monocytes can either promote resolution of inflammation or contribute to tissue injury.