Rose bengal-integrated electrospun polyacrylonitrile nanofibers for photodynamic inactivation of bacteria
Rose bengal-integrated electrospun polyacrylonitrile nanofibers for photodynamic inactivation of bacteria
复制标题
玫瑰红集成电纺聚丙烯腈纳米纤维用于光动力灭活细菌
DOI：
10.1039/d2va00166g
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Yan, Fei
中科院分区：
文献类型：
--
作者：
Dong, Xiuli;Mitchell, Dionne G.;Garcia Cervantes, Martha Y.;Chitara, Basant;Yang, Liju;Yan, Fei
采用静电纺丝法制备了不同负载量的光敏剂玫瑰红（RB）对聚丙烯腈（PAN）纳米纤维进行光动力灭活。我们的研究结果表明，在介质中的离子强度并没有显着影响RB集成的静电纺丝PAN纳米纤维（RBiEPN），它可以有效地释放RB在磷酸盐缓冲液（PBS），生理盐水（0.85%NaCl），和去离子水释放RB。然而，介质的pH值显着影响RB的释放。在较高pH下，在PBS中释放更大量的RB（RB释放：pH 9.0 > pH 7.4 > pH 5.0）。RBiEPN表现出对革兰氏阴性大肠杆菌（E. coli）和革兰氏阳性枯草芽孢杆菌（B. subtilis）细菌在白色光照射下进行。对细菌细胞，特别是B，具有强效和即时的抗菌功效。枯草杆菌。含有0.33重量% RB的RBiEPN证明了对B的完全细菌杀灭。枯草芽孢杆菌和E.在30 s和40 min内，对数值分别为5.76和5.94。在RBiEPN的存在下，在细菌细胞的白色光处理后，检查细胞内活性氧物质（iROS）的产生。发现iROS的量与RBiEPN的抗微生物功效之间存在显著相关性。高抗微生物功效可归因于若干因素，例如RBiEPN的包封效率、负载能力和RB释放行为、白色光的存在和iROS的产生。总之，通过共混静电纺丝将光敏剂容易地掺入聚合物纳米纤维中为水消毒提供了可行的策略。
Electrospun polyacrylonitrile (PAN) nanofibers integrated with different loadings of the photosensitizer rose bengal (RB) were synthesized for photodynamic inactivation of bacteria. Our results suggest that the ionic strength in the medium does not significantly affect the RB release from the RB-integrated electrospun PAN nanofibers (RBiEPNs), which could release RB effectively in phosphate-buffered saline (PBS), physiological saline (0.85% NaCl), and deionized H2O. However, the pH of the medium significantly influenced the release of RB. A larger amount of RB was released in PBS at a higher pH (RB release: pH 9.0 > pH 7.4 > pH 5.0). The RBiEPNs depicted high antimicrobial efficacy against both Gram-negative Escherichia coli (E. coli) and Gram-positive Bacillus subtilis (B. subtilis) bacteria under white light irradiation. The antimicrobial efficacy was potent and immediate against the bacterial cells, especially B. subtilis. The RBiEPNs containing 0.33 wt% RB demonstrated complete bacterial kills for B. subtilis and E. coli cells with log reductions of 5.76 and 5.94 in 30 s and 40 min, respectively. The generation of intracellular reactive oxygen species (iROS) was examined after white light treatment of the bacterial cells in the presence of the RBiEPNs. A significant correlation was found between the amount of iROS and the antimicrobial efficacy of the RBiEPNs. The high antimicrobial efficacy could be attributed to several factors, such as the encapsulation efficiency, loading capacity, and RB release behavior of the RBiEPNs, the presence of white light, and the generation of iROS. Taken together, the facile incorporation of a photosensitizer into polymeric nanofibers via blend electrospinning offers a feasible strategy for water disinfection.