Cytokinin and auxin inhibit abscisic acid-induced stornatal closure by enhancing ethylene production in Arabidopsis
Cytokinin and auxin inhibit abscisic acid-induced stornatal closure by enhancing ethylene production in Arabidopsis
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DOI：
10.1093/jxb/erj193
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发表时间：
2006-01-01
影响因子：
6.9
通讯作者：
Hasezawa, Selichiro
中科院分区：
文献类型：
--
作者：
Tanaka, Yoko;Sano, Toshio;Hasezawa, Selichiro
细胞分裂素和生长素是参与植物生长和发育各个方面的主要植物激素。这些植物激素还可以拮抗脱落酸 (ABA) 对气孔运动的影响，并影响乙烯生物合成。由于乙烯对 ABA 诱导的气孔关闭具有拮抗作用，因此研究了这些植物激素对 ABA 的拮抗作用是通过乙烯生物合成介导的可能性。细胞分裂素 6-苄基腺嘌呤 (BA) 和生长素 1-萘乙酸 (NAA) 均以与应用乙烯前体 1-氨基环丙烷-1-羧酸 (ACC) 后类似的方式拮抗 ABA 诱导的气孔关闭。然而，当乙烯信号传导、感知或生物合成被阻断时，这些影响就会被抵消。由于气孔孔径是通过保卫细胞体积的变化来调节的，因此发现 ABA 的应用会减少保卫细胞原生质体 (GCP) 的体积。结果发现，BA、NAA 或 ACC 的施用完美地补偿了 WT 植物中 ABA 施用造成的 GCP 体积的减少。上述观察表明，细胞分裂素和生长素通过调节乙烯合成来抑制 ABA 诱导的气孔关闭，并且乙烯抑制 ABA 诱导的保卫细胞渗透压降低。
Cytokinins and auxins are major phylohormones involved in various aspects of plant growth and development. These phytohormones are also known to antagonize the effects of abscisic acid (ABA) on stomatal movement, and to affect ethylene biosynthesis. As ethylene has an antagonistic effect on ABA-induced stomatal closure, the possibility that the antagonistic effects of these phytohormones on ABA were mediated through ethylene biosynthesis was investigated. Both the cytokinin, 6-benzyladenine (BA), and the auxin, 1-naphthaleneacetic acid (NAA), antagonized ABA-induced stomatal closure in a manner similar to that following application of the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid (ACC). However, these effects were negated when ethylene signalling, perception, or biosynthesis were blocked. As stomatal aperture is regulated by changes in guard cell volume, ABA application was found to reduce the volume of the guard cell protoplasts (GCP). It was found that BA, NAA, or ACC application compensated perfectly for the reduction in GCP volume by ABA application in WT plants. The above observations suggest that cytokinins and auxins inhibit ABA-induced stomatal closure through the modulation of ethylene blosynthesis, and that ethylene inhibits the ABA-induced reduction of osmotic pressure in the guard cells.