Developmental relationship between dopa decarboxylase, dopamine acetyltransferase, and ecdysone in Drosophila.
Developmental relationship between dopa decarboxylase, dopamine acetyltransferase, and ecdysone in Drosophila.
复制标题
果蝇多巴脱羧酶、多巴胺乙酰转移酶和蜕皮激素之间的发育关系。
DOI：
10.1016/0012-1606(80)90412-1
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发表时间：
1980
影响因子：
2.7
通讯作者：
Wright,TR
中科院分区：
文献类型：
--
作者：
Marsh,JL;Wright,TR
确定多巴脱羧酶 (DDC) 和多巴胺乙酰转移酶 (DAT) 的发育概况，并与蜕皮激素的发育概况进行比较。观察到 DDC 活性的五个峰值，对应于果蝇发育、胚胎孵化、第一次和第二次蜕皮、蛹形成和成虫羽化的五个主要转变点。还观察到 DAT 活性的显着变化，尽管该模式与发育阶段的变化没有很好的相关性。 DDC 表达与蛹形成时蜕皮激素浓度的增加相关，而胚胎孵化和成虫羽化时 DDC 的峰值与蜕皮激素没有时间相关性。温度敏感的无蜕皮激素突变体 (ecd1) 在限制性温度下可逆地阻断蜕皮激素的产生 [Garen, A., Kauvar, L., 和 Lepesant, J. A. (1977).Proc.纳特。阿卡德。科学。 USA74,5099–5133]被用来证明化蛹幼虫中DDC活性增加20倍是激素依赖性的。结果发现，ecd1突变体未能阻止蛹中期蜕皮激素浓度的增加。
The developmental profiles of dopa decar☐ylase (DDC) and dopamine acetyltransferase (DAT) were determined and compared with that of ecdysone. Five peaks of DDC activity are observed corresponding to the five major transition points inDrosophiladevelopment, embryonic hatching, first and second molts, puparium formation, and adult eclosion. Marked changes in DAT activity are also observed, although the pattern does not correlate well with changes in developmental stages. DDC expression is correlated with increasing ecdysone concentration at puparium formation, while the peaks of DDC at embryonic hatching and adult eclosion are not temporally correlated with ecdysone. The temperature-sensitive ecdysoneless mutant (ecd1) which is reversibly blocked in ecdysone production at restrictive temperatures [Garen, A., Kauvar, L., and Lepesant, J. A. (1977).Proc. Nat. Acad. Sci. USA74,5099–5133] was used to show that the 20-fold increase in DDC activity in pupating larvae is hormone dependent. It was found that theecd1mutant failed to block the midpupal increase in ecdysone concentration.