EXTRAATRIAL EXPRESSION OF THE GENE FOR ATRIAL-NATRIURETIC-FACTOR
EXTRAATRIAL EXPRESSION OF THE GENE FOR ATRIAL-NATRIURETIC-FACTOR
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DOI：
10.1073/pnas.83.18.6697
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发表时间：
1986-09-01
影响因子：
11.1
通讯作者：
LEWICKI, J
中科院分区：
文献类型：
--
作者：
GARDNER, DG;DESCHEPPER, CF;LEWICKI, J
心房利钠因子（ANF）是一组最初从心房分离的肽，对血压、肾功能和盐平衡具有许多重要作用。在目前的研究中，在某些心房外组织的大鼠的心钠素基因的表达已被检查提取的心钠素蛋白的放射免疫分析和印迹杂交，核酸酶S1分析，和引物延伸分析的心钠素mRNA。在心室、肺和脑垂体中检测到ANF肽和mRNA，其水平通常≤心房水平的1%。心房外组织中的ANF转录物似乎与心房中合成的ANF转录物非常相似。它们是聚腺苷酰化的，全长相等（950-1050个核苷酸），并具有相同的5“末端。次级转录起始位点定位在初级起始位点上游约80个碱基对，在心房中使用，在其他组织中使用程度较低。ANF转录本存在于从心尖到基底的整个心室中以及隔膜和心室游离壁中。转录本在左心室和室间隔中比在右心室中更普遍。使用各种抗大鼠ANF抗体的免疫细胞化学定位ANF免疫反应性的心房肌细胞;心室肌细胞，特别是沿着心室腔的内皮表面;肺泡周围细胞在肺;和垂体的促性腺激素产生细胞。这些数据表明，ANF基因表达的能力超出心房组织，虽然在大大降低的水平，并可能建议替代，也许是副内分泌，在这些组织中的肽的功能。
Atrial natriuretic factor (ANF) is a group of peptides, originally isolated from the cardiac atria, that have a number of important effects on blood pressure, renal function, and salt balance. In the current study, expression of the ANF gene in certain extra-atrial tissues of the rat has been examined by radioimmunoassay of extracted ANF protein and by blot-hybridization, nuclease S1 analysis, and primer-extension analysis of the ANF mRNA. ANF peptides and mRNA were detected in cardiac ventricles, lung, and pituitary gland at levels generally .ltoreq.1% those of cardiac atria. The ANF transcripts in extra-atrial tissue appear to be very similar to those synthesized in the atria. They are polyadenylylated, are equivalent in overall length (950-1050 nucleotides), and have identical 5'' termini. A secondary transcription start site mapping approximately 80 base pairs upstream from the primary start site is employed in atria and to a lesser extent in other tissues. The ANF transcript is present throughout the cardiac ventricles from apex to base and in the septum as well as the ventricular free walls. The transcript is more prevalent in the left ventricle and interventricular septum than in the right ventricle. Immunocytochemistry using various anti-rat ANF antibodies localized ANF immunoreactivity to the atrial myocytes; the ventricular myocytes, particularly along the endothelial surface of the ventricular chamber; perialveolar cells in the lung; and the gonadotropin-producing cells of the pituitary. The data indicate that the capacity for ANF gene expression extends beyond atrial tissue, albeit at much reduced levels, and may suggest alternative, perhaps paraendocrine, functions for the peptide in these tissues.