Characteristics of clinical and environmental vanM-carrying vancomycin-resistant enterococci isolates from an infected patient
Characteristics of clinical and environmental vanM-carrying vancomycin-resistant enterococci isolates from an infected patient
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来自感染患者的携带 vanM 的耐万古霉素肠球菌分离株的临床和环境特征。
DOI：
10.1016/j.ijantimicag.2018.01.003
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发表时间：
2018-06-01
影响因子：
10.8
通讯作者：
Cui, Wei
中科院分区：
文献类型：
--
作者：
Zhang, Gensheng;Lin, Xiuhui;Cui, Wei
vanM是一种罕见的糖肽耐药基因，于2006年首次在中国上海的一株粪肠球菌分离株（Efm-HS0661）中被发现，自2011年以来在该城市占主导地位。2014年，在中国杭州的重症监护病房（ICU），从一名患者的血液中分离出一种携带面包车的粪肠杆菌。对患者直肠拭子和环境表面的进一步监测筛查发现大量vanm阳性屎肠杆菌。这些分离株（包括1株来自血液，1株来自直肠拭子和43株来自环境样本的代表性分离株）被分为4种脉冲场凝胶电泳（PFGE）模式和2种序列类型（ST78和ST564）。PCR扩增和序列分析表明，这些分离株的vanM基因周围的遗传结构与原携带vanM的分离株Efm-HS0661相似。本研究强调了在上海以外的中国另一个城市的ICU中由携带面包车的粪肠杆菌引起的感染和环境污染的出现。(c) 2018 Elsevier B.V.和国际化学疗法学会。版权所有。
vanM, an uncommon glycopeptide resistance gene, was first identified in an Enterococcus faecium isolate (Efm-HS0661) from Shanghai, China, in 2006 and has been predominant in this city since 2011. A vanM-carrying E. faecium was isolated from the bloodstream of a patient in an intensive care unit (ICU) in Hangzhou, China, in 2014. Further surveillance screening of a rectal swab and environmental surfaces of the patient yielded a large number of vanM-positive E. faecium. These isolates (including 1 from the bloodstream, 1 from the rectal swab and 43 representative isolates from environmental samples) were classified into four pulsed-field gel electrophoresis (PFGE) patterns and two sequence types (ST78 and ST564). PCR amplification and sequence analysis indicated that the genetic structure surrounding the vanM gene of these isolates was similar to that of the original vanM-carrying isolate Efm-HS0661. This study highlights the emergence of infections and environmental contamination caused by vanM-carrying E. faecium in an ICU of another Chinese city outside of Shanghai. (c) 2018 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.