Intermittent ischemia produces a cumulative depletion of mitochondrial adenine nucleotides in the isolated perfused rat heart.
Intermittent ischemia produces a cumulative depletion of mitochondrial adenine nucleotides in the isolated perfused rat heart.
复制标题
间歇性缺血会导致离体灌注大鼠心脏中线粒体腺嘌呤核苷酸的累积消耗。
DOI：
10.1161/01.res.66.2.302
复制
发表时间：
1990
影响因子：
20.1
通讯作者：
Zwischenberger,JB
中科院分区：
文献类型：
--
作者：
Asimakis,GK;Sandhu,GS;Conti,VR;Sordahl,LA;Zwischenberger,JB
本研究的目的是确定重复性心肌缺血是否会导致线粒体腺嘌呤核苷酸的累积丢失。离体灌流大鼠心脏进行连续或间歇缺血。一个单一的5分钟的持续缺血不会导致线粒体腺嘌呤核苷酸池的显着减少，一个单一的10分钟的缺血导致约17%的减少。接下来，将缺血前和30分钟连续缺血心脏的线粒体的腺嘌呤核苷酸含量与经历间歇性缺血的两组心脏（两组均接受总共30分钟的缺血）进行比较。一组接受3次10分钟的缺血发作，间隔5分钟的再灌注（3 x 10分钟的间歇性缺血）;另一组接受6次5分钟的缺血发作，间隔5分钟的灌注（6 x 5分钟的间歇性缺血）。缺血前和30分钟连续缺血心脏的线粒体腺嘌呤核苷酸含量（以纳摩尔/纳摩尔细胞色素a表示）分别为14.7 +/- 0.6和8.0 +/- 0.4。3 × 10分钟间歇缺血组的线粒体腺嘌呤核苷酸含量（8.5 ± 0.5）与30分钟连续缺血组无显著差异。6 × 5分钟间歇缺血组线粒体腺嘌呤核苷酸含量（11.0 ± 0.6）显著高于30分钟连续缺血组和3 × 10分钟间歇缺血组（p <0.05）。（250字处删节）
The purpose of the present study was to determine if repetitive myocardial ischemia would result in the cumulative loss of mitochondrial adenine nucleotides. Isolated perfused rat hearts were subjected to continuous or intermittent ischemia. A single 5-minute period of continuous ischemia did not result in a significant decrease in the mitochondrial adenine nucleotide pool; a single 10-minute period of ischemia resulted in a decrease of approximately 17%. Next, the adenine nucleotide content of mitochondria from preischemic and 30-minute continuous ischemic hearts was compared with two groups of hearts undergoing intermittent ischemia (both groups receiving a total of 30 minutes of ischemia). One group received three 10-minute episodes of ischemia interrupted by 5-minute periods of reperfusion (3 x 10-minute intermittent ischemia); the other intermittent ischemic group received six 5-minute episodes of ischemia interrupted by 5-minute periods of perfusion (6 x 5-minute intermittent ischemia). The mitochondrial adenine nucleotide content (expressed as nanomoles per nanomole cytochrome a) for the preischemic and 30-minute continuous ischemic hearts was 14.7 +/- 0.6 and 8.0 +/- 0.4, respectively. The mitochondrial adenine nucleotide content of the 3 x 10-minute intermittent ischemia group (8.5 +/- 0.5) was not significantly different from the 30-minute continuous ischemic group. The mitochondrial adenine nucleotide content of the 6 x 5-minute intermittent ischemia group (11.0 +/- 0.6) was significantly larger than that of the 30-minute continuous and the 3 x 10-minute intermittent ischemia groups (p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)