MICROINJECTION OF LIVE SPERMATOZOA INTO SEA URCHIN EGGS
MICROINJECTION OF LIVE SPERMATOZOA INTO SEA URCHIN EGGS
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DOI：
10.1016/0014-4827(62)90006-x
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发表时间：
1962-01-01
影响因子：
3.7
通讯作者：
HIRAMOTO, Y
中科院分区：
文献类型：
--
作者：
HIRAMOTO, Y
将海胆、Psammechinus microtuberculatus、Paracentrotus lividus 和 Clypeaster japonicus 的活精子显微注射到同一物种的卵中。通过注射精子，未受精卵从未发生激活。卵子内的精子的外观与卵子外的精子的外观相同。他们经常不时地表现出痉挛性的动作。注入精子的卵子可以通过体外受精而受精。在卵子受精时，注射的精子的行为没有发生变化。与对照卵相比，在授精前注射精子的卵以及授精后的卵中，第一次卵裂时的多精分裂百分比较高。有人提出，如果原生质从外部被激活，注射到卵子原生质中的一些精子可以参与有丝分裂过程。
Live spermatozoa of the sea urchins, Psammechinus microtuberculatus, Paracentrotus lividus and Clypeaster japonicus were microinjected into the eggs of the same species. Activation never occurred in unfertilized eggs by the injection of spermatozoa. The appearance of the spermatozoon inside the egg was the same as that outside the egg. They often exhibited spasmodic motions from time to time. The egg injected with spermatozoa could be fertilized by insemination from outside. No change in the behavior of the injected spermatozoa was recognized at the time of fertilization of the egg. Percentage of polyspermic divisions in the first cleavage was high in the eggs injected with spermatozoa before insemination as well as in the eggs after insemination as compared with control eggs. It was suggested that some of the spermatozoa injected into the egg protoplasm can participate in the mitotic process if the protoplasm is activated from outside.