EFFECT OF GLYCEROL MONOLAURATE ON BACTERIAL-GROWTH AND TOXIN PRODUCTION
EFFECT OF GLYCEROL MONOLAURATE ON BACTERIAL-GROWTH AND TOXIN PRODUCTION
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DOI：
10.1128/aac.36.3.626
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发表时间：
1992-03-01
影响因子：
4.9
通讯作者：
NOVICK, RP
中科院分区：
文献类型：
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作者：
SCHLIEVERT, PM;DERINGER, JR;NOVICK, RP
甘油单月桂酸酯（GML）是一种天然存在的表面活性剂，可用作卫生棉条和伤口敷料的添加剂，以减少某些细菌毒素介导的疾病的发生率。 进行体外研究以评估GML对潜在致病细菌的生长和毒素产生的影响。 GML在10至20 μ g/ml的浓度下抑制A、B、F和G群链球菌的临床分离株的生长。 外毒素的产生，包括热原性外毒素和溶血素的产生，通过低于生长抑制和生长抑制的GML浓度而减少。 中毒性休克综合征和烫伤皮肤综合征患者的金黄色葡萄球菌菌株的生长在100 - 300 μ g/ml的GML存在下被抑制或延迟。 GML的生长抑制可以通过脂肪酶的产生来克服。 S.当GML浓度低于抑制生长所需的浓度时，金黄色葡萄球菌溶血素、中毒性休克综合征毒素1和脱落毒素A的产生受到抑制。 结果与S.金黄色葡萄球菌试验中获得。人类 大肠杆菌和沙门氏菌的生长不受GML的影响，但沙门氏菌的生长受GML的影响。minillonRe突变体对生长抑制活性敏感。 E.大肠杆菌细胞也不受GML的影响，但E.大肠杆菌溶血素增加。 产链球菌致热外毒素A的E. coli克隆不受GML的影响。 这些研究表明，GML在阻断或延迟致病性革兰氏阳性菌产生外毒素方面是有效的。
Glycerol monolaurate (GML) is a naturally occurring surfactant that has potential use as an additive to tampons and wound dressings to reduce the incidence of certain bacterial toxin-mediated illnesses. In vitro studies were undertaken to evaluate the effect of GML on the growth of and toxin production by potentially pathogenic bacteria. GML inhibited the growth of clinical isolates of group A, B, F, and G streptococci at concentrations of 10 to 20-mu-g/ml. Exotoxin production, including that of pyrogenic exotoxins and hemolysins, was reduced by concentrations of GML that were below those inhibitory for growth as well as growth inhibitory. The growth of Staphylococcus aureus strains from patients with toxic shock syndrome and scalded skin syndrome was inhibited or delayed in the presence of 100 to 300-mu-g of GML per ml. Growth inhibition by GML could be overcome by the production of lipase. S. aureus elaboration of hemolysin, toxic shock syndrome toxin 1, and exfoliative toxin A was inhibited at GML concentrations below those necessary to inhibit growth. Results similar to those for S. aureus were obtained in tests of S. hominis. Escherichia coli growth and Salmonella minnesota growth were unaffected by GML, but an S. minnesota Re mutant was susceptible to growth-inhibitory activity. Endotoxin release into the medium from E. coli cells was also unaffected by GML, but the release or activity of E. coli hemolysin was increased by GML. Streptococcal pyrogenic exotoxin A production by an E. coli clone was not affected by GML. These studies indicate that GML is effective in blocking or delaying the production of exotoxins by pathogenic gram-positive bacteria.