Screening of promising chemotherapeutic candidates from plants against human adult T-cell leukemia/lymphoma (III)
Screening of promising chemotherapeutic candidates from plants against human adult T-cell leukemia/lymphoma (III)
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DOI：
10.1007/s11418-013-0747-2
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发表时间：
2013-10-01
影响因子：
3.3
通讯作者：
Kinjo, Junei
中科院分区：
文献类型：
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作者：
Nakano, Daisuke;Ishitsuka, Kenji;Kinjo, Junei
成人T细胞白血病/淋巴瘤(ATL)是由人类T细胞嗜淋性病毒I型(HTLV-I)引起的一种成熟外周T淋巴细胞的恶性肿瘤。在我们之前的论文中，我们从162种植物中筛选出214种提取物，以阐明其抗HTLV-I感染T细胞系的增殖活性。在这项研究中，进一步测试了61科182种植物的245种提取物对两个HTLV-I感染T细胞系(MT-1和MT-2)的抑制作用。在所测试的245个提取物中，分别有52个和60个提取物对MT-1和MT-2细胞显示出强大的抗增殖作用。其中，2个提取物对两种细胞均表现出较强的抑制活性(EC50值为0.1~1mUg/mL；+)，7个提取物表现出中等抑制活性(EC50值为1~10mUg/mL；++)，43个提取物表现出较弱的抑制活性(EC50值为10~100mUg/mL；+)，其余提取物对MT-1细胞均无抑制作用(EC50值为100mUg/mL；-)。另一方面，10个提取物表现出中等抑制活性，48个提取物表现出弱抑制活性，其余提取物对MT-2细胞没有任何抑制活性。番荔枝和鳞片番荔枝的地上部分提取物具有最强的抑制活性，通过活性导向分级法从其提取物中分离出3个阿朴吗啡生物碱作为活性成分。
Adult T-cell leukemia/lymphoma (ATL) is a malignancy of mature peripheral T lymphocytes caused by human T-cell lymphotropic virus type I (HTLV-I). In our previous paper, 214 extracts from 162 plants were screened to elucidate the anti-proliferative principles against HTLV-I-infected T-cell lines. In this study, 245 extracts from 182 plants belonging to 61 families were further tested against two HTLV-I-infected T-cell lines (MT-1 and MT-2). Potent anti-proliferative effects were exhibited against MT-1 and MT-2 cells by 52 and 60 of the 245 extracts tested, respectively. Of these, two extracts showed strong inhibitory activity (EC50 values 0.1-1 mu g/mL; +++) against both cells, 7 extracts showed moderate inhibitory activity (EC50 values 1-10 mu g/mL; ++), and 43 extracts showed weak inhibitory activity (EC50 values 10-100 mu g/mL; +), whereas the remaining extracts did not show any activity (EC50 values > 100 mu g/mL; -) against MT-1 cells. On the other hand, 10 extracts showed moderate inhibitory activit and, 48 extracts showed weak inhibitory activity, whereas the remaining extracts did not show any activity against MT-2 cells. Extracts from the aerial parts of Annona reticulata and A. squamosa showed the most potent inhibitory activity and three aporphine alkaloids were isolated from their extracts as the active principles by activity-guided fractionation.