The phagocytic activity of human first trimester extravillous trophoblast
The phagocytic activity of human first trimester extravillous trophoblast
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DOI：
10.1093/humrep/13.10.2941
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发表时间：
1998-10-01
影响因子：
6.1
通讯作者：
Manyonda, IT
中科院分区：
文献类型：
--
作者：
Choy, MY;Manyonda, IT
以前曾有人提出，人胎盘中的吞噬活性仅限于巨噬细胞谱系的细胞。然而，早期的研究受到了原代滋养层细胞培养物中固有的细胞的缺乏和较差的活力、自身具有吞噬活性的其他细胞类型的污染、缺乏可靠的滋养层标志物以及可用于明确证明颗粒物质内化的方法的局限性的阻碍。我们已经克服了这些局限性，通过使用：（i）DNA转染，以提供无限供应的纯滋养层细胞系;（ii）人胎盘催乳素作为滋养层特有的标志物;和（iii）共聚焦显微镜，以明确地证明吞噬材料的细胞内位置。我们发现未转染的原代培养绒毛外滋养层细胞以及细胞系都具有吞噬绵羊红细胞、金黄色葡萄球菌和面包酵母细胞的能力，并且这种活性被细胞松弛素B和4 ℃培养物抑制。滋养层细胞中的吞噬活性不如专职吞噬细胞中的吞噬活性。在发生组织重塑的生理和病理情况下，例如牙周韧带中的快速周转或炎症期间，上皮细胞和其他不被认为是专职吞噬细胞的细胞主动吞噬细胞外基质的组分。我们推测，人滋养层细胞的吞噬作用可能在滋养层细胞侵入蜕膜的广泛组织重塑中发挥重要作用。
It has been suggested previously that phagocytic activity in the human placenta is confined to cells of the macrophage lineage. However, earlier studies were hampered by the paucity and poor viability of cells inherent in primary trophoblast cell cultures, contamination by other cell types which themselves have phagocytic activity, lack of reliable markers of trophoblasts, and by limitations of methods available to demonstrate unequivocally the internalization of particulate material. We have overcome these limitations by using: (i) DNA transfection to provide unlimited supplies of pure trophoblast cell lines; (ii) human placental lactogen as a marker unique to trophoblast; and (iii) confocal microscopy to demonstrate unequivocally the intracellular locality of phagocytosed material. We found that both untransfected primary culture extravillous trophoblast cells, as well as the cell lines, had the capacity to phagocytose sheep red blood cells, Staphylococcus aureus and baker's yeast cells, and that this activity was inhibited by cytochalasin B and by culture at 4 degrees C. Phagocytic activity in trophoblast cells was less avid than that seen in a professional phagocyte. In physiological and pathological situations where tissue remodelling occurs, such as the rapid turnover in the periodontal ligament or during inflammation, epithelial cells and other cells that are not considered professional phagocytes actively phagocytose components of the extracellular matrix. We postulate that phagocytosis by human trophoblasts may play an important role in the extensive tissue remodelling that occurs during trophoblastic invasion of the decidua.