Gallic Acid Attenuates Postoperative Intra-Abdominal Adhesion by Inhibiting Inflammatory Reaction in a Rat Model.
Gallic Acid Attenuates Postoperative Intra-Abdominal Adhesion by Inhibiting Inflammatory Reaction in a Rat Model.
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没食子酸通过抑制大鼠模型的炎症反应来减轻术后腹腔粘连
DOI：
10.12659/msm.908550
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发表时间：
2018-02-09
期刊：
影响因子：
--
通讯作者：
Sun X
中科院分区：
文献类型：
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作者：
Wei G;Wu Y;Gao Q;Shen C;Chen Z;Wang K;Yu J;Li X;Sun X
腹内粘连是腹部手术后最常见的并发症之一。目前治疗腹内粘连的效果并不理想。本研究采用腹腔粘连大鼠模型，探讨没食子酸对腹腔手术后腹腔粘连的预防和治疗作用。材料/方法将实验大鼠随机分为假手术组、对照组、壳聚糖组和3个不同浓度没食子酸组。除假手术组外，其余大鼠均行盲肠磨损诱导粘连。术后第1天起，未食子酸组大鼠每天灌胃不同浓度的未食子酸2 ml。术后第7天处死大鼠，肉眼观察腹腔内粘连程度。采用天狼星红染色法测定损伤腹膜组织间胶原沉积量。ELISA法检测血清白细胞介素-6 （IL-6）、肿瘤坏死因子（TNF-α）、转化生长因子-β (TGF-β)水平。Western blot法检测损伤大鼠腹膜或粘连组织中NF-κB磷酸化水平。结果与对照组比较，大剂量没食子酸处理大鼠腹腔内粘连评分明显降低，炎症和纤维化程度也明显降低。没食子酸显著降低血清IL-6、TNF-α和TGF-β1水平。高没食子酸组NF-κB磷酸化显著降低。结论没食子酸通过抑制炎症反应和纤维生成抑制大鼠术后腹腔粘连的形成。没食子酸是一种很有前途的预防腹内粘连的药物。
Background Intra-abdominal adhesion is one of the most common complications after abdominal surgery. The efficacy of current treatments for intra-abdominal adhesion is unsatisfactory. In this study, we investigated the effect of gallic acid on the prevention and treatment of intra-abdominal adhesions after abdominal surgery using an intra-abdominal adhesion rat model. Material/Methods The experimental rats were randomly divided into the sham operation group, the control group, the chitosan group, and 3 gallic acid groups of different concentrations. All rats except those in the sham operation group received cecal abrasion to induce adhesion. From the first postoperative day, the rats in the gallic acid groups were administered different concentrations of gallic acid in a 2-ml gavage daily. All rats were sacrificed on postoperative day 7, and the degree of intra-abdominal adhesion was evaluated by the naked eye. The amount of collagen deposited between the injured peritoneal tissues was assessed by Sirius red staining. Serum levels of interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor-β (TGF-β) were measured by ELISA. Western blot was used to detect the level of NF-κB phosphorylation in the injured peritoneal or adhesion tissues of the rats. Results Compared with the control group, the scores of intra-abdominal adhesions in the rats treated with larger doses of gallic acid were significantly decreased, and the degree of inflammation and fibrosis was also significantly decreased. Gallic acid significantly reduced IL-6, TNF-α, and TGF-β1 serum levels. NF-κB phosphorylation in the higher gallic acid groups was significantly reduced. Conclusions Gallic acid inhibits the formation of postoperative intra-abdominal adhesions in rats by inhibiting the inflammatory reaction and fibrogenesis. Gallic acid is a promising drug for preventing intra-abdominal adhesions.