Effect of local administration of platelet-rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model
Effect of local administration of platelet-rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model
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DOI：
10.1002/micr.30263
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发表时间：
2018-03-01
期刊：
影响因子：
2.1
通讯作者：
Yamazaki, Masashi
中科院分区：
文献类型：
--
作者：
Ikumi, Akira;Hara, Yuki;Yamazaki, Masashi
本研究旨在评估雪旺细胞（SCs）在局部给予自体富血小板血浆（PRP）后的神经再生效果和行为。方法选用日本大白兔28只。左侧肢体产生15mm坐骨神经缺损。切除的神经用作反向自体神经。这些兔子被随机分为两组。A组（n=10）只行神经移植。B组（n=18）行局部PRP给药神经移植。以右肢为对照（C组，n=28）。各组按移植后4周和12周的评估期平均分为2个亚组。通过电生理评价、肌肉湿重、组织学评价和多重免疫荧光染色研究PRP的再生作用。结果4周时，B组移植部分再生轴突平均直径（2.020.22 m）显著大于A组（1.89±0.16 m）。12周时，B组远端再生轴突数量（9017 +/- 2224/mm(2)）明显高于a组（4955 +/- 3117/mm(2)）。电生理评价和肌肉湿重无显著差异。在免疫组织学评估中，B组活化SCs的数量（188 +/- 90/mm(2)）比a组（117 +/- 51/mm(2)）增加的程度更大。结论局部给药可增加再生轴突直径和远端再生轴突数量。PRP加速SC在体内的增殖。
This study aimed to evaluate the nerve regenerative effect and behavior of Schwann cells (SCs) on local administration of autologous platelet-rich plasma (PRP).MethodsTwenty-eight Japanese white rabbits were used. A 15-mm sciatic nerve defect was created on the left limb. The resected nerve was used as a reverse autologous nerve. The rabbits were randomly divided into two groups. In group A (n=10), only nerve grafting was performed. In group B (n=18), nerve grafting was performed with local PRP administration. Right limbs were used as control (group C, n=28). The rabbits in each group were equally divided into two subgroups based on the evaluation period of 4 and 12 weeks after grafting. Electrophysiological evaluation, muscle wet-weight, histological evaluation, and multiple immunofluorescence staining were performed to investigate the regenerative effect of PRP.ResultsThe mean regenerative axon diameter of the graft portion in group B (2.020.22-m) was significantly larger than that in group A (1.89 +/- 0.16-m) at 4 weeks. The regenerative axon number at the distal portion showed a greater increase in group B (9017 +/- 2224/mm(2)) than in group A (4955 +/- 3117/mm(2)) at 12 weeks. Electrophysiological evaluation and muscle wet-weight revealed no significant differences. On immunohistological evaluation, the number of activated SCs increased to a larger extent in group B (188 +/- 90/mm(2)) than in group A (117 +/- 51/mm(2)).ConclusionsLocal PRP administration increases the regenerative axon diameter and the regenerative axon number at the distal portion. PRP accelerates SC proliferation in vivo.