Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis.
Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis.
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成年大鼠睾丸中阿黑皮质素原信使 RNA 的表征和定位。
DOI：
10.1126/science.6740329
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Krieger,DT
中科院分区：
文献类型：
--
作者：
Pintar,JE;Schachter,BS;Herman,AB;Durgerian,S;Krieger,DT
北方印迹分析的总RNA和多聚腺苷酸RNA分离成年大鼠睾丸表明，阿黑皮素原（POMC）样信使RNA分子存在于这些提取物。睾丸POMC信使RNA的长度与杏仁核和中脑POMC信使RNA相当，并且似乎比下丘脑和垂体前叶和中叶中发现的POMC信使RNA短至少200个核苷酸。原位杂交结果表明，POMC信使RNA位于Leydig细胞，这是唯一的睾丸细胞，含有免疫组织化学POMC衍生肽。这些结果表明，POMC的局部合成发生在睾丸。
Northern blot analysis of total RNA and polyadenylated RNA isolated from adult rat testes showed that a proopiomelanocortin (POMC)-like messenger RNA molecule is present in these extracts. The testicular POMC messenger RNA is comparable in length to amygdala and midbrain POMC messenger RNA and appears to be at least 200 nucleotides shorter than POMC messenger RNA found in the hypothalamus and anterior and intermediate lobes of the pituitary gland. Hybridization in situ showed that POMC messenger RNA is located in Leydig cells, which are the only testicular cells that contain immunostainable POMC-derived peptides. These results suggest that local synthesis of POMC occurs in the testis.