Binding of transmissible gastroenteritis coronavirus to brush border membrane sialoglycoproteins
Binding of transmissible gastroenteritis coronavirus to brush border membrane sialoglycoproteins
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DOI：
10.1128/jvi.77.21.11846-11848.2003
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发表时间：
2003-11-01
影响因子：
5.4
通讯作者：
Herrler, G
中科院分区：
文献类型：
--
作者：
Schwegmann-Wessels, C;Zimmer, G;Herrler, G
猪传染性胃肠炎冠状病毒（TGEV）是一种引起哺乳仔猪肠道感染的病原体。TGEV的肠嗜性与病毒表面蛋白S的唾液酸结合活性有关。在这里，我们表明，在猪肠刷状缘膜蛋白，TGEV识别的粘蛋白型糖蛋白指定MGP在唾液酸依赖的方式。用仔猪小肠冰冻切片进行病毒结合试验，发现TGEV与产生粘蛋白的杯状细胞结合。缺乏唾液酸结合活性的非肠道致病性突变病毒不能与MGP结合并附着于杯状细胞。我们的研究结果表明MGP在TGEV的肠道致病性中的作用。
Transmissible gastroenteritis coronavirus (TGEV) is a porcine pathogen causing enteric infections that are lethal for suckling piglets. The enterotropism of TGEV is connected with the sialic acid binding activity of the viral surface protein S. Here we show that, among porcine intestinal brush border membrane proteins, TGEV recognizes a mucin-type glycoprotein designated MGP in a sialic acid-dependent fashion. Virus binding assays with cryosections of the small intestine from a suckling piglet revealed the binding of TGEV to mucin-producing goblet cells. A nonenteropathogenic mutant virus that lacked a sialic acid binding activity was unable to bind to MGP and to attach to goblet cells. Our results suggest a role of MGP in the enteropathogenicity of TGEV.