NADPH Thioredoxin Reductase C Is Localized in Plastids of Photosynthetic and Nonphotosynthetic Tissues and Is Involved in Lateral Root Formation in Arabidopsis
NADPH Thioredoxin Reductase C Is Localized in Plastids of Photosynthetic and Nonphotosynthetic Tissues and Is Involved in Lateral Root Formation in Arabidopsis
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DOI：
10.1105/tpc.111.092304
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发表时间：
2012-04-01
期刊：
影响因子：
11.6
通讯作者：
Javier Cejudo, Francisco
中科院分区：
文献类型：
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作者：
Kirchsteiger, Kerstin;Ferrandez, Julia;Javier Cejudo, Francisco
质体是存在于光合和非光合植物组织中的细胞器。虽然它是众所周知的，硫氧还蛋白依赖的氧化还原调节是必不可少的叶叶绿体功能，很少有人知道的非光合组织，不能使用光作为还原力的直接来源的质体中的氧化还原调节。因此，问题仍然是氧化还原调节是否在非光合质体功能，以及它是如何与植物生长的叶绿体整合。在这里，我们表明，NADPH-硫氧还蛋白还原酶C（NTRC），以前报道为专有的绿色组织，也表达在非光合组织的拟南芥，在那里它是本地化的质体。此外，我们表明，NTRC参与维持氧化还原平衡的质体也在非光合器官。为了测试光合和非光合组织的质体之间的关系，获得了转基因植物的氧化还原稳态恢复专门在叶或根，通过表达NTRC的器官特异性启动子的控制下，在ntrc突变体。我们的研究结果表明，功能齐全的根淀粉体是不够的根，或叶，生长，但功能齐全的叶绿体是必要的，足以支持野生型的根生长和侧根形成率。
Plastids are organelles present in photosynthetic and nonphotosynthetic plant tissues. While it is well known that thioredoxin-dependent redox regulation is essential for leaf chloroplast function, little is known of the redox regulation in plastids of nonphotosynthetic tissues, which cannot use light as a direct source of reducing power. Thus, the question remains whether redox regulation operates in nonphotosynthetic plastid function and how it is integrated with chloroplasts for plant growth. Here, we show that NADPH-thioredoxin reductase C (NTRC), previously reported as exclusive to green tissues, is also expressed in nonphotosynthetic tissues of Arabidopsis thaliana, where it is localized to plastids. Moreover, we show that NTRC is involved in maintaining the redox homeostasis of plastids also in nonphotosynthetic organs. To test the relationship between plastids of photosynthetic and nonphotosynthetic tissues, transgenic plants were obtained with redox homeostasis restituted exclusively in leaves or in roots, through the expression of NTRC under the control of organ-specific promoters in the ntrc mutant. Our results show that fully functional root amyloplasts are not sufficient for root, or leaf, growth, but fully functional chloroplasts are necessary and sufficient to support wild-type rates of root growth and lateral root formation.