Acute and long-term stability studies of deoxy hemoglobin and characterization of ascorbate-induced modifications
Acute and long-term stability studies of deoxy hemoglobin and characterization of ascorbate-induced modifications
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DOI：
10.1021/js980221r
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发表时间：
1999-01-01
影响因子：
3.8
通讯作者：
Looker, DL
中科院分区：
文献类型：
--
作者：
Kerwin, BA;Akers, MJ;Looker, DL
研究了在不同氧分压下，抗坏血酸与重组血红蛋白(rHb1.1)的反应。在15000ppm(1.5%)剩余氧存在下，抗坏血酸/氧介导的反应导致自氧化速率增加，β-珠蛋白被修饰，氧亲和力增加，最大Hill系数降低。观察到的其中一个对β-珠蛋白的修饰是在其N端增加了一个72Da。详细的表征表明，该修饰为咪唑烷酮型结构。将血红蛋白溶液彻底脱氧以
The reaction of ascorbate with recombinant hemoglobin (rHb1.1) in the presence of differing partial pressures of oxygen was studied. In the presence of 15 000 ppm (1.5%) residual oxygen, ascorbate/oxygen-mediated reactions resulted in an increased rate of autoxidation, modification of the beta-globin, increased oxygen affinity and decreased maximum Hill coefficient. One of the observed modifications to the beta-globin was a`72 Da addition to its N-terminus. Detailed characterization indicates the modification was an imidazolidinone type structure. Thorough deoxygenation of the hemoglobin solution to