Genome-wide analysis of gene expression profiles associated with cell cycle transitions in growing organs of Arabidopsis
Genome-wide analysis of gene expression profiles associated with cell cycle transitions in growing organs of Arabidopsis
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DOI：
10.1104/pp.104.053884
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发表时间：
2005-06-01
期刊：
影响因子：
7.4
通讯作者：
Vuylsteke, M
中科院分区：
文献类型：
--
作者：
Beemster, GTS;De Veylder, L;Vuylsteke, M
器官生长是由组成细胞在达到成熟之前通过随后的增殖和扩张阶段的进展引起的。我们结合运动学分析，流式细胞术，和微阵列分析，以表征细胞周期调控过程中的叶1和2的拟南芥（拟南芥）。运动学分析表明，表皮增殖，直到第12天，此后，细胞扩大，直到第19天，当叶子达到成熟。流式细胞术显示，从细胞分裂速率下降到细胞扩增结束的时间内发生核内复制。用6 k-cDNA微阵列对10个时间点的分析表明，生长阶段之间的转变密切反映在mRNA表达数据中。随后对三个主要阶段的全基因组微阵列分析使我们能够将已知的细胞周期基因分为三大类：组成型表达、增殖和抑制。与发表的表达数据的比较，从根区对应于类似的发育阶段，并从同步细胞培养支持这种分类，使我们能够确定一个高置信度的131个增殖基因。这些基因中的大多数具有M期依赖性表达模式，除了许多已知的细胞周期相关基因外，至少有90个基因是未知的或以前与增殖无关。
Organ growth results from the progression of component cells through subsequent phases of proliferation and expansion before reaching maturity. We combined kinematic analysis, flowcytometry, and microarray analysis to characterize cell cycle regulation during the growth process of leaves 1 and 2 of Arabidopsis ( Arabidopsis thaliana). Kinematic analysis showed that the epidermis proliferates until day 12; thereafter, cells expand until day 19 when leaves reach maturity. Flowcytometry revealed that endoreduplication occurs from the time cell division rates decline until the end of cell expansion. Analysis of 10 time points with a 6k- cDNA microarray showed that transitions between the growth stages were closely reflected in the mRNA expression data. Subsequent genome- wide microarray analysis on the three main stages allowed us to categorize known cell cycle genes into three major classes: constitutively expressed, proliferative, and inhibitory. Comparison with published expression data obtained from root zones corresponding to similar developmental stages and from synchronized cell cultures supported this categorization and enabled us to identify a high confidence set of 131 proliferation genes. Most of those had an M phase- dependent expression pattern and, in addition to many known cell cycle- related genes, there were at least 90 that were unknown or previously not associated with proliferation.