The nucleotide sequence of a nematode vitellogenin gene.
The nucleotide sequence of a nematode vitellogenin gene.
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线虫卵黄原基因的核苷酸序列。
DOI：
10.1093/nar/13.19.7129
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发表时间：
1985
影响因子：
14.9
通讯作者：
Blumenthal,T
中科院分区：
文献类型：
--
作者：
Spieth,J;Denison,K;Zucker,E;Blumenthal,T
这种线虫名为Caenorhabditiselegans，它包含一个由6个基因组成的家族，这些基因编码卵黄蛋白原。在这里，我们报告了其中一个基因的完整核苷酸序列，vit5。该基因指定一个4869个核苷酸的mRNA，包括5 ‘端9个碱基的非翻译区和3 ’端51个碱基的非翻译区。vit5含有4个短内含子，总长度为218bp。预测的卵黄原蛋白yp170A分子量为186430。在它的N端，它显然与脊椎动物的卵黄蛋白原有关。然而，vit5编码的蛋白质不包含与脊椎动物卵黄蛋白phosvitin相关的富含丝氨酸的序列。事实上，线虫蛋白的氨基酸组成与不含磷光素的脊椎动物蛋白非常相似。vit5具有高度不对称的密码子选择字典。这些有利密码子不同于其他生物的有利密码子，但它们是高表达秀丽隐杆线虫基因的特征。在基因的5 '端附近，对稀有密码子的强选择不那么强；稀有密码子在前54 bp的频率是其后4.8 kb的15倍。
The nematode,Caenorhabditiselegans, contains a family of six genes that code for vitellogenins. Here we report the complete nucleotide sequence of one of these genes,vit-5. The gene specifies a mRNA of 4869 nucleotides, including untranslated regions of 9 bases at the 5′ end and 51 bases at the 3′ end.Vit-5contains four short introns totalling 218 bp. The predicted vitellogenin, yp170A, has a molecular weight of 186,430. At its N terminus it is clearly related to the vitellogenins of vertebrates. However, thevit-5-encoded protein does not contain a serine-rich sequence related to the vertebrate vitellin, phosvitin. In fact, the amino acid composition of the nematode protein is very similar to that of the vertebrate protein without phosvitin.Vit-5has a highly asymmetric codon choice dictionary. The favored codons are different from those favored in other organisms, but are characteristic of highly expressedC.elegansgenes. The strong selection against rare codons is not as great near the 5′ end of the gene; rare codons are 15 times more frequent within the first 54 bp than in the next 4.8 kb.