Ischemic preconditioning reduces transplanted submandibular gland injury.
Ischemic preconditioning reduces transplanted submandibular gland injury.
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DOI：
10.1016/j.jss.2012.02.066
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
N. Yang;Liang Shi;Yan Zhang;C. Ding;X. Cong;F. Fu;Li-Ling Wu;G. Yu
中科院分区：
文献类型：
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作者：
N. Yang;Liang Shi;Yan Zhang;C. Ding;X. Cong;F. Fu;Li-Ling Wu;G. Yu
缺血预处理（IPC）可减轻多器官、多物种的缺血/再灌注（I/R）损伤。然而，IPC对移植的下颌下腺的影响尚不清楚。为探讨缺血预处理（IPC）对兔下颌下腺移植的保护作用及其机制，采用舌动脉阻断10 min，再灌注10 min的方法进行IPC。雄性家兔随机分为对照组、移植组和IPC组（每组6只）。在再灌注后1、12和24 h测定唾液分泌、氧化应激、促炎细胞因子水平和前列腺增生相关蛋白水平。缺血预处理组再灌注24 h唾液分泌量明显减少（P < 0.01）。移植腺体中肿瘤坏死因子-α、白细胞介素-1 β和活性氧的mRNA水平以及丙二醛（MDA）和髓过氧化物酶活性显著升高，超氧化物歧化酶活性降低。IPC治疗后上述改变均明显减轻（P均< 0.05）。IPC处理组再灌注后1和12 h腺泡细胞凋亡减少，Bax蛋白表达减少，Bcl-2蛋白表达增加（均P < 0.05）。结论IPC通过减轻炎症反应、减轻氧化应激和抗凋亡过程保护兔移植下颌下腺的分泌功能。
BACKGROUNDIschemic preconditioning (IPC) can reduce ischemia/reperfusion (I/R) injury in multiple organs and species. However, the effect of IPC on transplanted submandibular glands remains unknown. We explored the protection of IPC in transplanted submandibular glands in the rabbit and the underlying mechanism.METHODSIPC was performed by clamping the lingual artery for 10 min, with 10 min of reperfusion before transplantation. Male rabbits were randomly divided into control, transplantation, and IPC groups (n = 6 each). Saliva secretion, oxidative stress, pro-inflammatory cytokine levels, and apoptosis-related protein levels were determined at 1, 12, and 24 h after reperfusion.RESULTSSalivary flow was significantly increased at 12 h and decreased at 24 h in the transplanted glands. IPC treatment prevented the reduced saliva secretion at 24 h after reperfusion (P < 0.01). The mRNA levels of tumor necrosis factor-α, interleukin-1β, and reactive oxygen species, as well as malondialodehyde (MDA) and myeloperoxidase activity, were significantly increased and superoxide dismutase activity was decreased in the transplanted glands. However, these changes were all attenuated with IPC treatment (all P < 0.05). Also, acinar cell apoptosis and Bax protein expression were decreased and Bcl-2 protein expression was increased in the IPC-treated glands at 1 and 12 h after reperfusion (all P < 0.05).CONCLUSIONSIPC protects the secretory function of transplanted submandibular gland in the rabbit by reducing the inflammatory response, attenuating oxidative stress, and an anti-apoptosis process.