Adenosine Inhibits Histamine‐Induced Phosphoinositide Hydrolysis Mediated via Pertussis Toxin‐Sensitive G Protein in Human Astrocytoma Cells
Adenosine Inhibits Histamine‐Induced Phosphoinositide Hydrolysis Mediated via Pertussis Toxin‐Sensitive G Protein in Human Astrocytoma Cells
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腺苷抑制人星形细胞瘤细胞中百日咳毒素敏感 G 蛋白介导的组胺诱导的磷酸肌醇水解
DOI：
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发表时间：
1991
影响因子：
4.7
通讯作者：
H. Nakanishi
中科院分区：
文献类型：
--
作者：
N. Nakahata;M. T. Abe;I. Matsuoka;Tomoyuki Ono;H. Nakanishi
在1321 N1人星形细胞瘤细胞中检测腺苷对磷酸肌醇水解的影响。腺苷、L-N6-苯基异丙基腺苷（l-PIA）和5-（N-乙基甲酰氨基）腺苷（NECA）以浓度依赖性方式抑制组胺刺激的磷酸肌醇蓄积。腺苷类似物抑制磷酸肌醇蓄积的效力顺序为l-PIA>腺苷> NECA，这一发现表明A1类腺苷受体参与抑制。腺苷受体拮抗剂8-苯基茶碱可阻断l-PIA导致的磷酸肌醇蓄积减少。刺激A1类腺苷受体可抑制异丙肾上腺素刺激的环AMP蓄积以及组胺诱导的磷酸肌醇蓄积。这两种抑制作用均通过用百日咳毒素[胰岛活化蛋白（IAP）]预处理细胞来阻断。l-PIA还抑制鸟苷5′-（γ-硫代）三磷酸（GTPγ）刺激的膜制备物中肌醇磷酸的积累，8-苯基茶碱拮抗该抑制。在IAP处理的膜中，l-PIA不能抑制GTPγS诱导的肌醇磷酸的积累。从兔脑中纯化的Gi/G 0在膜制备物中以浓度依赖性方式抑制GTPγS刺激的肌醇磷酸积累。这些结果表明，Ar类腺苷受体的刺激与IAP敏感性G蛋白相互作用，导致人星形细胞瘤细胞中磷脂酶C和腺苷酸环化酶的抑制。
The effect of adenosine on phosphoinositide hydrolysis was examined in 1321N1 human astrocytoma cells. Adenosine, L‐N6‐phenylisopropyladenosine (l‐PIA), and 5‐ (N‐ethylcarboxamido)adenosine (NECA) inhibited histaminc‐stimulated accumulation of inositol phosphates in a concentration‐dependent manner. The potency order of adenosine analogues for inhibition of inositol phosphate accumulation was l‐PIA > adenosine > NECA, a finding indicating that A1‐class adenosine receptors are involved in the inhibition. The reduction in inositol phosphate accumulation by l‐PIA was blocked by an adenosine receptor antagonist, 8‐phenyltheophylline. Stimulation of A1‐class adenosine receptors inhibited isoproterenol‐stimulated cyclic AMP accumulation as well as histamine‐induced inositol phosphate accumulation. Both inhibitory effects were blocked by pretreatment of the cells with pertussis toxin [islet‐activating protein (IAP)]. l‐PIA also inhibited guanosine 5′‐(γ‐thio)triphosphate (GTPγ)‐stimulated accumulation of inositol phosphates in membrane preparations, and 8‐phenyltheophylline antagonized the inhibition. l‐PIA could not inhibit GTPγS‐induced accumulation of inositol phosphates in IAP‐treated membranes. Gi/G0, purified from rabbit brain, inhibited GTPγS‐stimulated accumulation of inositol phosphates in a concentration‐dependent manner in membrane preparations. These results suggest that stimulation of Ar class adenosine receptors interacts with the IAP‐sensitive G protein(s), resulting in the inhibitions of phospholipase C as well as adenylate cyclase in human astrocytoma cells.
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Go α 和三种类型的 Gi α 在大肠杆菌中表达后的纯化和表征。
DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Linder,ME;Ewald,DA;Miller,RJ;Gilman,AG
通讯作者：
Gilman,AG
通过腺苷 A1 受体调节 GH3 细胞功能。
DOI：
10.1042/bj2550069
发表时间：
1988
期刊：
The Biochemical journal
影响因子：
--
作者：
Delahunty,TM;Cronin,MJ;Linden,J
通讯作者：
Linden,J
腺苷酸环化酶调节成分的鸟嘌呤核苷酸激活位点。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Northup,JK;Smigel,MD;Gilman,AG
通讯作者：
Gilman,AG
影响因子：
13.9
作者：
S. Snyder
通讯作者：
S. Snyder
鸟嘌呤核苷酸介导对大脑皮质膜磷脂酶 C 活性的刺激和抑制作用。
DOI：
10.1042/bj2610245
发表时间：
1989
期刊：
The Biochemical journal
影响因子：
--
作者：
Litosch,I
通讯作者：
Litosch,I