Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus.
Serum Metabolomic Profiling of Piglets Infected with Virulent Classical Swine Fever Virus.
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感染强毒猪瘟病毒仔猪的血清代谢组学分析
DOI：
10.3389/fmicb.2017.00731
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发表时间：
2017
影响因子：
5.2
通讯作者：
Tu C
中科院分区：
文献类型：
--
作者：
Gong W;Jia J;Zhang B;Mi S;Zhang L;Xie X;Guo H;Shi J;Tu C
猪瘟是一种高度传染性的猪瘟，给全球养猪业造成了巨大的经济损失。这项研究的目的是确定小分子代谢物是否与脑脊液的发病有关。采用超高效液相色谱/电喷雾飞行时间质谱仪(UPLC/ESI-Q-TOF/MS)结合多元统计分析方法，对感染CSFV石门株仔猪的血清代谢组学进行了分析。在感染CSFV的仔猪中，在感染后第3天和第7天，与几个关键代谢途径有关的代谢产物发生了变化，包括色氨酸分解代谢和犬尿氨酸途径、苯丙氨酸代谢、脂肪酸和脂肪代谢、三羧酸和尿素循环、支链氨基酸代谢和核苷酸代谢。参与能量代谢的几条途径，包括脂肪酸生物合成和β氧化、支链氨基酸代谢和三羧酸循环，都受到了显著的抑制。还观察到与肠道微生物区系有关的几种代谢物的变化。代谢组学分析表明CSFV与宿主肠道微生物群的相互作用在脑脊液的发育过程中起着重要作用。
Classical swine fever (CSF) is a highly contagious swine infectious disease and causes significant economic losses for the pig industry worldwide. The objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of CSF. Birefly, serum metabolomics of CSFV Shimen strain-infected piglets were analyzed by ultraperformance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) in combination with multivariate statistical analysis. In CSFV-infected piglets at days 3 and 7 post-infection changes were found in metabolites associated with several key metabolic pathways, including tryptophan catabolism and the kynurenine pathway, phenylalanine metabolism, fatty acid and lipid metabolism, the tricarboxylic acid and urea cycles, branched-chain amino acid metabolism, and nucleotide metabolism. Several pathways involved in energy metabolism including fatty acid biosynthesis and β-oxidation, branched-chain amino acid metabolism, and the tricarboxylic acid cycle were significantly inhibited. Changes were also observed in several metabolites exclusively associated with gut microbiota. The metabolomic profiles indicate that CSFV-host gut microbiome interactions play a role in the development of CSF.