A simplified method for the quantitative determination of testosterone-estradiol-binding globulin activity in human plasma.
A simplified method for the quantitative determination of testosterone-estradiol-binding globulin activity in human plasma.
复制标题
定量测定人血浆中睾酮雌二醇结合球蛋白活性的简化方法。
DOI：
--
复制
发表时间：
1972
影响因子：
5.8
通讯作者：
W. Rosner
中科院分区：
文献类型：
--
作者：
W. Rosner
描述了一种快速简便的测定人血浆中睾酮-雌二醇结合球蛋白的方法。结合蛋白用二氢睾酮（DHT）饱和，用50%硫酸铵沉淀，结合DHT的质量由已知的添加类固醇的比活性确定。正常值，以M-g DHT结合/100 ml血清表示：男性，0.93±。0.06 (SEM);女性：1.85±0.13；妊娠，12.4±0.67。在男性肝硬化患者和甲亢患者中观察到该数值升高。在戒酒者和患有糖尿病或高血压的孕妇中观察到正常值。（/临床内分泌杂志34:983,1972）
A rapid and simple method for the measurement of testosterone-estradiol-binding globulin in human plasma is described. The bind- ing protein is saturated with dihydrotestosterone (DHT), precipitated with 50% ammonium sul- fate, and the mass of bound DHT determined from the known specific activity of the added steroid. Normal values, expressed as M-g DHT bound/100 ml serum are: men, 0.93 ±. 0.06 (SEM); women, 1.85 ± 0.13; pregnancy, 12.4 ± 0.67. Elevated values were observed in men with cirrhosis of the liver and patients with thyrotoxi- cosis. Normal values were observed in abstinent alcoholics and in pregnant subjects with diabetes or hypertension. (/ Clin Endocrinol Metab 34: 983, 1972)