Proton nuclear magnetic resonance study of a selectively deuterated mouse monoclonal antibody: use of two-dimensional homonuclear Hartmann-Hahn spectroscopy.
Proton nuclear magnetic resonance study of a selectively deuterated mouse monoclonal antibody: use of two-dimensional homonuclear Hartmann-Hahn spectroscopy.
复制标题
选择性氘化小鼠单克隆抗体的质子核磁共振研究：使用二维同核哈特曼-哈恩光谱。
DOI：
10.1093/oxfordjournals.jbchem.a122858
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发表时间：
1989
影响因子：
2.7
通讯作者：
Y. Arata
中科院分区：
文献类型：
--
作者：
K. Kato;Y. Nishimura;M. Waelchli;Y. Arata
本文报道了一种选择性氚代小鼠抗丹磺酰单抗的~1H核磁共振研究。二维同核Hartmann-Hahn(2D-HOHAHA)光谱被发现可以有效地建立抗体分子中His残基的C2-H和C4-H质子之间的连接性。结果表明：1)即使在大的蛋白质如抗体的情况下，也可以观察到位于灵活环境中的氨基酸残基的HOHAHA峰；2)氚标记有效地降低了自旋驰豫的效率，并使观察到的HOHAHA交叉峰的数量增加成为可能。提示2D-HOHAHA也可用于获得有关抗体分子柔性部分的信息。
A 1H NMR study of a selectively deuterated mouse anti-dansyl monoclonal antibody is reported. Two-dimensional homonuclear Hartmann-Hahn (2D-HOHAHA) spectroscopy was found to be effective for establishing the connectivity between the C2-H and C4-H protons of His residues in the antibody molecule. It has been concluded that 1) even in the case of large proteins such as an antibody, HOHAHA peaks can be observed for amino acid residues that are located in a flexible environment, and 2) deuterium labeling is effective in reducing the efficiency of spin relaxation and makes it possible to increase the number of observed HOHAHA cross peaks. It was suggested that 2D-HOHAHA can also be used to obtain information concerning the flexible parts of antibody molecules.