Induction of lateral root structure formation on petunia roots: A novel effect of GMI1000 Ralstonia solanacearum infection impaired in Hrp mutants.
Induction of lateral root structure formation on petunia roots: A novel effect of GMI1000 Ralstonia solanacearum infection impaired in Hrp mutants.
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DOI：
10.1094/mpmi-19-0597
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发表时间：
2006-06
期刊：
影响因子：
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通讯作者：
L. Zolobowska;F. Van Gijsegem
中科院分区：
文献类型：
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作者：
L. Zolobowska;F. Van Gijsegem
青枯雷尔氏菌（Ralstonia solanacearum）是一种土传植物病原菌，通过根部侵入寄主。与许多革兰氏阴性细菌植物病原体一样，R。青枯菌Hrp III型分泌系统对于细菌与植物的相互作用是必不可少的;然而，涉及疾病表达的相关机制在很大程度上是未知的。在这项工作中，我们研究了感染的影响，由R。青枯菌GMI 1000和Hrp突变体对矮牵牛植物根系的影响。野生型和突变体菌株都通过抑制侧根伸长和引起根尖肿胀来干扰矮牵牛根构型的发育。此外，GMI 100而不是Hrp突变体诱导新的根侧结构（RLS）的形成。这种能力是由其他的，但不是所有的，R。测试的青枯菌菌株。像侧根一样，这些新的结构是由周细胞的分裂产生的，然而，这些细胞表现出异常的形态。这些RLS是允许广泛细菌增殖的有效定殖位点。然而，它们不是导致细菌在整个植物中系统传播的细菌维管入侵所必需的，这表明，相反，它们可能在R.青枯菌生活史
Ralstonia solanacearum is a soilborne plant pathogen that invades its host via roots. As in many gram-negative bacterial plant pathogens, the R. solanacearum Hrp type III secretion system is essential for interactions of the bacterium with plants; however, the related mechanisms involved in disease expression are largely unknown. In this work, we examined the effects of infection by R. solanacearum GMI1000 and Hrp mutants on the root system of petunia plants. Both the wild-type and mutant strains disturbed the petunia root architecture development by inhibiting lateral root elongation and provoking swelling of the root tips. In addition, GMI100 but not the Hrp mutants induced the formation of new root lateral structures (RLS). This ability is shared by other, but not all, R. solanacearum strains tested. Like lateral roots, these new structures arise from divisions of pericycle founder cells which, nevertheless, exhibit an abnormal morphology. These RLS are efficient colonization sites allowing extensive bacterial multiplication. However, they are not required for the bacterial vascular invasion that leads to the systemic spread of the bacterium through the whole plant, indicating that, instead, they might play a role in the rhizosphere-related stages of the R. solanacearum life cycle.