A technical consideration concerning the removal of oocyte vitelline membranes for patch clamp recording.
A technical consideration concerning the removal of oocyte vitelline membranes for patch clamp recording.
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关于去除卵母细胞卵黄膜以进行膜片钳记录的技术考虑。
DOI：
10.1016/j.bbrc.2004.09.162
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发表时间：
2004
影响因子：
3.1
通讯作者：
Myeon
中科院分区：
文献类型：
--
作者：
Myeon
我们开发了一种有效的方法来去除非洲爪蟾卵母细胞的卵黄膜以进行膜片钳记录。使用卵母细胞作为模型的功能研究提供了对离子通道的生物学特征和生理特性的深入了解。本文描述了方法上的修改。这种修饰的重要特点是使用蛋白酶处理去除卵母细胞的卵黄膜。该方法简单，产生的卵母细胞在记录过程中保持健康状态。
We have developed an efficient method for removing the vitelline membrane of Xenopus oocytes for patch clamp recording. Functional studies using oocytes as models provide insights into the biological profiles and physiological properties of ion channels. A methodological modification is described in this paper. The important feature of this modification is that protease treatment is used to remove the oocyte’s vitelline membrane. This method is simple and the oocytes produced remain in a healthy state during the recording process.
DOI：
10.1073/pnas.92.20.9422
发表时间：
1995-09-26
影响因子：
11.1
作者：
PLANELLSCASES, R;FERRERMONTIEL, AV;MONTAL, M
通讯作者：
MONTAL, M